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| {{STRUCTURE_2gr8| PDB=2gr8 | SCENE= }} | | {{STRUCTURE_2gr8| PDB=2gr8 | SCENE= }} |
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| '''Hia 1022-1098'''
| | ===Hia 1022-1098=== |
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| ==Overview==
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| Autotransporter proteins are defined by the ability to drive their own secretion across the bacterial outer membrane. The Hia autotransporter of Haemophilus influenzae belongs to the trimeric autotransporter subfamily and mediates bacterial adhesion to the respiratory epithelium. In this report, we present the crystal structure of the C-terminal end of Hia, corresponding to the entire Hia translocator domain and part of the passenger domain (residues 992-1098). This domain forms a beta-barrel with 12 transmembrane beta-strands, including four strands from each subunit. The beta-barrel has a central channel of 1.8 nm in diameter that is traversed by three N-terminal alpha-helices, one from each subunit. Mutagenesis studies demonstrate that the transmembrane portion of the three alpha-helices and the loop region between the alpha-helices and the neighboring beta-strands are essential for stability of the trimeric structure of the translocator domain, and that trimerization of the translocator domain is a prerequisite for translocator activity. Overall, this study provides important insights into the mechanism of translocation in trimeric autotransporters.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16688217}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16688217 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16688217}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Protein secretion]] | | [[Category: Protein secretion]] |
| [[Category: Trimeric autotransporter]] | | [[Category: Trimeric autotransporter]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:25:31 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:06:08 2008'' |