2yyk: Difference between revisions

Revision as of 14:48, 28 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:2yyk.png

Template:STRUCTURE 2yyk

Crystal structure of the mutant of HpaB (T198I, A276G, and R466H)Crystal structure of the mutant of HpaB (T198I, A276G, and R466H)

Template:ABSTRACT PUBMED 17804419

About this StructureAbout this Structure

2YYK is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the oxygenase component (HpaB) of the 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8., Kim SH, Hisano T, Takeda K, Iwasaki W, Ebihara A, Miki K, J Biol Chem. 2007 Nov 9;282(45):33107-17. Epub 2007 Sep 5. PMID:17804419

Crystallization and preliminary X-ray analysis of the oxygenase component (HpaB) of 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8., Kim SH, Miyatake H, Hisano T, Iwasaki W, Ebihara A, Miki K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jul 1;63(Pt, 7):556-9. Epub 2007 Jun 11. PMID:17620709

Page seeded by OCA on Mon Jul 28 14:48:29 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2yyk.jpg|left|200px]]
+{{Seed}}
+[[Image:2yyk.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_2yyk|  PDB=2yyk  |  SCENE=  }}
-'''Crystal structure of the mutant of HpaB (T198I, A276G, and R466H)'''
+===Crystal structure of the mutant of HpaB (T198I, A276G, and R466H)===
-==Overview==
+<!--
-The 4-hydroxyphenylacetate (4HPA) 3-monooxygenase is involved in the initial step of the 4HPA degradation pathway and catalyzes 4HPA hydroxylation to 3,4-dihydroxyphenylacetate. This enzyme consists of two components, an oxygenase (HpaB) and a reductase (HpaC). To understand the structural basis of the catalytic mechanism of HpaB, crystal structures of HpaB from Thermus thermophilus HB8 were determined in three states: a ligand-free form, a binary complex with FAD, and a ternary complex with FAD and 4HPA. Structural analysis revealed that the binding and dissociation of flavin are accompanied by conformational changes of the loop between beta5 and beta6 and of the loop between beta8 and beta9, leading to preformation of part of the substrate-binding site (Ser-197 and Thr-198). The latter loop further changes its conformation upon binding of 4HPA and obstructs the active site from the bulk solvent. Arg-100 is located adjacent to the putative oxygen-binding site and may be involved in the formation and stabilization of the C4a-hydroperoxyflavin intermediate.
+The line below this paragraph, {{ABSTRACT_PUBMED_17804419}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 17804419 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_17804419}}
 ==About this Structure==
@@ Line 20: / Line 24: @@
 ==Reference==
 Crystal structure of the oxygenase component (HpaB) of the 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8., Kim SH, Hisano T, Takeda K, Iwasaki W, Ebihara A, Miki K, J Biol Chem. 2007 Nov 9;282(45):33107-17. Epub 2007 Sep 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17804419 17804419]
+Crystallization and preliminary X-ray analysis of the oxygenase component (HpaB) of 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8., Kim SH, Miyatake H, Hisano T, Iwasaki W, Ebihara A, Miki K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jul 1;63(Pt, 7):556-9. Epub 2007 Jun 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17620709 17620709]
 [[Category: 4-hydroxyphenylacetate 3-monooxygenase]]
 [[Category: Single protein]]
@@ Line 36: / Line 42: @@
 [[Category: Two-component flavin diffusible monooxygenase]]
 [[Category: Unligand form]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 19:42:05 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:48:29 2008''