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{{STRUCTURE_2fte|  PDB=2fte  |  SCENE=  }}  
{{STRUCTURE_2fte|  PDB=2fte  |  SCENE=  }}  


'''Bacteriophage HK97 Expansion Intermediate IV'''
===Bacteriophage HK97 Expansion Intermediate IV===




==Overview==
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Maturation of the bacteriophage HK97 capsid from a precursor (Prohead II) to the mature state (Head II) involves a 60 A radial expansion. The mature particle is formed by 420 copies of the major capsid protein organized on a T = 7 laevo lattice with each subunit covalently crosslinked to two neighbors. Well-characterized pH 4 expansion intermediates make HK97 valuable for investigating quaternary structural dynamics. Here, we use X-ray crystallography and cryo-EM to demonstrate that in the final transition in maturation (requiring neutral pH), pentons in Expansion Intermediate IV (EI-IV) reversibly sample 14 A translations and 6 degrees rotations relative to a fixed hexon lattice. The limit of this trajectory corresponds to the Head II conformation that is secured at this extent only by the formation of the final class of covalent crosslinks. Mutants that cannot crosslink or EI-IV particles that have been rendered incapable of forming the final crosslink remain in the EI-IV state.
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{{ABSTRACT_PUBMED_17098191}}


==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM., Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE, Structure. 2006 Nov;14(11):1655-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17098191 17098191]
Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM., Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE, Structure. 2006 Nov;14(11):1655-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17098191 17098191]
Control of crosslinking by quaternary structure changes during bacteriophage HK97 maturation., Gan L, Conway JF, Firek BA, Cheng N, Hendrix RW, Steven AC, Johnson JE, Duda RL, Mol Cell. 2004 Jun 4;14(5):559-69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15175152 15175152]
[[Category: Enterobacteria phage hk97]]
[[Category: Enterobacteria phage hk97]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Expansion intermediate]]
[[Category: Expansion intermediate]]
[[Category: Hk97]]
[[Category: Hk97]]
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