3bjh: Difference between revisions

Revision as of 14:23, 28 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:3bjh.png

Template:STRUCTURE 3bjh

Soft-SAD crystal structure of a pheromone binding protein from the honeybee Apis mellifera L.Soft-SAD crystal structure of a pheromone binding protein from the honeybee Apis mellifera L.

Template:ABSTRACT PUBMED 14594955

About this StructureAbout this Structure

3BJH is a Single protein structure of sequence from Apis mellifera. This structure supersedes the now removed PDB entry 1r5r. Full crystallographic information is available from OCA.

ReferenceReference

Sulfur single-wavelength anomalous diffraction crystal structure of a pheromone-binding protein from the honeybee Apis mellifera L., Lartigue A, Gruez A, Briand L, Blon F, Bezirard V, Walsh M, Pernollet JC, Tegoni M, Cambillau C, J Biol Chem. 2004 Feb 6;279(6):4459-64. Epub 2003 Oct 31. PMID:14594955

Structural Basis of the Honey Bee PBP Pheromone and pH-induced Conformational Change., Pesenti ME, Spinelli S, Bezirard V, Briand L, Pernollet JC, Tegoni M, Cambillau C, J Mol Biol. 2008 Apr 27;. PMID:18508083

Page seeded by OCA on Mon Jul 28 14:23:52 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:3bjh.jpg|left|200px]]
+{{Seed}}
+[[Image:3bjh.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_3bjh|  PDB=3bjh  |  SCENE=  }}
-'''Soft-SAD crystal structure of a pheromone binding protein from the honeybee Apis mellifera L.'''
+===Soft-SAD crystal structure of a pheromone binding protein from the honeybee Apis mellifera L.===
-==Overview==
+<!--
-Pheromone binding proteins (PBPs) are small helical proteins ( approximately 13-17 kDa) present in several sensory organs from moth and other insect species. They are involved in the transport of pheromones from the sensillar lymph to the olfactory receptors. We report here the crystal structure of a PBP (Amel-ASP1) originating from the honey-bee (Apis mellifera) antennae and expressed as recombinant protein in the yeast Pichia pastoris. Crystals of Amel-ASP1 were obtained at pH 5.5 using the nano-drops technique of crystallization with a novel optimization procedure, and the structure was solved initially with the single-wavelength anomalous diffraction technique using sulfur anomalous dispersion. The structure of Amel-ASP1 has been refined at 1.6-A resolution. Its fold is roughly similar to that of other PBP/odorant binding proteins, presenting six helices and three disulfide bridges. Contrary to the PBPs from Bombyx mori (Sandler, B. H., Nikonova, L., Leal, W. S., and Clardy, J. (2000) Chem. Biol. 7, 143-151) and Leucophea maderae (Lartigue, A., Gruez, A., Spinelli, S., Riviere, S., Brossut, R., Tegoni, M., and Cambillau, C. (2003) J. Biol. Chem. 278, 30213-30218), the extended C terminus folds into the protein and forms a wall of the internal hydrophobic cavity. Its backbone groups establish two hydrogen bonds with a serendipitous ligand, n-butyl-benzene-sulfonamide, an additive used in plastics. This mode of binding might, however, mimic that used by one of the pheromonal blend components and illustrates the binding versatility of PBPs.
+The line below this paragraph, {{ABSTRACT_PUBMED_14594955}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 14594955 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_14594955}}
 ==About this Structure==
@@ Line 20: / Line 24: @@
 ==Reference==
 Sulfur single-wavelength anomalous diffraction crystal structure of a pheromone-binding protein from the honeybee Apis mellifera L., Lartigue A, Gruez A, Briand L, Blon F, Bezirard V, Walsh M, Pernollet JC, Tegoni M, Cambillau C, J Biol Chem. 2004 Feb 6;279(6):4459-64. Epub 2003 Oct 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14594955 14594955]
+Structural Basis of the Honey Bee PBP Pheromone and pH-induced Conformational Change., Pesenti ME, Spinelli S, Bezirard V, Briand L, Pernollet JC, Tegoni M, Cambillau C, J Mol Biol. 2008 Apr 27;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18508083 18508083]
 [[Category: Apis mellifera]]
 [[Category: Single protein]]
@@ Line 35: / Line 41: @@
 [[Category: Pheromone binding protein]]
 [[Category: Signal transduction]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:51:09 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:23:52 2008''