2go9: Difference between revisions

Revision as of 13:23, 28 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:2go9.png

Template:STRUCTURE 2go9

RRM domains 1 and 2 of Prp24 from S. cerevisiaeRRM domains 1 and 2 of Prp24 from S. cerevisiae

Template:ABSTRACT PUBMED 17320109

About this StructureAbout this Structure

2GO9 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA.

ReferenceReference

Structure and interactions of the first three RNA recognition motifs of splicing factor prp24., Bae E, Reiter NJ, Bingman CA, Kwan SS, Lee D, Phillips GN Jr, Butcher SE, Brow DA, J Mol Biol. 2007 Apr 13;367(5):1447-58. Epub 2007 Feb 7. PMID:17320109

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-'''RRM domains 1 and 2 of Prp24 from S. cerevisiae'''
+===RRM domains 1 and 2 of Prp24 from S. cerevisiae===
-==Overview==
+<!--
-The essential Saccharomyces cerevisiae pre-messenger RNA splicing protein 24 (Prp24) has four RNA recognition motifs (RRMs) and facilitates U6 RNA base-pairing with U4 RNA during spliceosome assembly. Prp24 is a component of the free U6 small nuclear ribonucleoprotein particle (snRNP) but not the U4/U6 bi-snRNP, and so is thought to be displaced from U6 by U4/U6 base-pairing. The interaction partners of each of the four RRMs of Prp24 and how these interactions direct U4/U6 pairing are not known. Here we report the crystal structure of the first three RRMs and the solution structure of the first two RRMs of Prp24. Strikingly, RRM 2 forms extensive inter-domain contacts with RRMs 1 and 3. These contacts occupy much of the canonical RNA-binding faces (beta-sheets) of RRMs 1 and 2, but leave the beta-sheet of RRM 3 exposed. Previously identified substitutions in Prp24 that suppress mutations in U4 and U6 spliceosomal RNAs cluster primarily in the beta-sheet of RRM 3, but also in a conserved loop of RRM 2. RNA binding assays and chemical shift mapping indicate that a large basic patch evident on the surface of RRMs 1 and 2 is part of a high affinity U6 RNA binding site. Our results suggest that Prp24 binds free U6 RNA primarily with RRMs 1 and 2, which may remodel the U6 secondary structure. The beta-sheet of RRM 3 then influences U4/U6 pairing through interaction with an unidentified ligand.
+The line below this paragraph, {{ABSTRACT_PUBMED_17320109}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 17320109 is the PubMed ID number.
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+{{ABSTRACT_PUBMED_17320109}}
 ==About this Structure==
-GO9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GO9 OCA].
+GO9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GO9 OCA].
 ==Reference==
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 [[Category: Rbd]]
 [[Category: Rrm]]
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