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| [[Image:2cj9.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2cj9| PDB=2cj9 | SCENE= }} | | {{STRUCTURE_2cj9| PDB=2cj9 | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF METHANOSARCINA BARKERI SERYL-TRNA SYNTHETASE COMPLEXED WITH AN ANALOG OF SERYLADENYLATE'''
| | ===CRYSTAL STRUCTURE OF METHANOSARCINA BARKERI SERYL-TRNA SYNTHETASE COMPLEXED WITH AN ANALOG OF SERYLADENYLATE=== |
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| ==Overview==
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| Methanogenic archaea possess unusual seryl-tRNA synthetase (SerRS), evolutionarily distinct from the SerRSs found in other archaea, eucaryotes and bacteria. The two types of SerRSs show only minimal sequence similarity, primarily within class II conserved motifs 1, 2 and 3. Here, we report a 2.5 A resolution crystal structure of the atypical methanogenic Methanosarcina barkeri SerRS and its complexes with ATP, serine and the nonhydrolysable seryl-adenylate analogue 5'-O-(N-serylsulfamoyl)adenosine. The structures reveal two idiosyncratic features of methanogenic SerRSs: a novel N-terminal tRNA-binding domain and an active site zinc ion. The tetra-coordinated Zn2+ ion is bound to three conserved protein ligands (Cys306, Glu355 and Cys461) and binds the amino group of the serine substrate. The absolute requirement of the metal ion for enzymatic activity was confirmed by mutational analysis of the direct zinc ion ligands. This zinc-dependent serine recognition mechanism differs fundamentally from the one employed by the bacterial-type SerRSs. Consequently, SerRS represents the only known aminoacyl-tRNA synthetase system that evolved two distinct mechanisms for the recognition of the same amino-acid substrate.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16675947}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16675947 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16675947}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Trna synthetase]] | | [[Category: Trna synthetase]] |
| [[Category: Zinc ion]] | | [[Category: Zinc ion]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 22:16:38 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:04:57 2008'' |