2qgr: Difference between revisions

Revision as of 12:41, 28 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:2qgr.png

Template:STRUCTURE 2qgr

Structure of the R178A mutant of delta PDZ DegS proteaseStructure of the R178A mutant of delta PDZ DegS protease

Template:ABSTRACT PUBMED 17981123

About this StructureAbout this Structure

2QGR is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Allosteric activation of DegS, a stress sensor PDZ protease., Sohn J, Grant RA, Sauer RT, Cell. 2007 Nov 2;131(3):572-83. PMID:17981123

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-'''Structure of the R178A mutant of delta PDZ DegS protease'''
+===Structure of the R178A mutant of delta PDZ DegS protease===
-==Overview==
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-Regulated intramembrane proteolysis is a method for transducing signals between cellular compartments. When protein folding is compromised in the periplasm of E. coli, the C termini of outer-membrane proteins (OMPs) bind to the PDZ domains of the trimeric DegS protease and activate cleavage of RseA, a transmembrane transcriptional regulator. We show here that DegS is an allosteric enzyme. OMP binding shifts the equilibrium from a nonfunctional state, in which the active sites are unreactive, to the functional proteolytic conformation. Crystallographic, biochemical, and mutagenic experiments show that the unliganded PDZ domains are inhibitory and suggest that OMP binding per se is sufficient to stabilize the relaxed conformation and activate DegS. OMP-induced activation and RseA binding are both positively cooperative, allowing switch-like behavior of the OMP-DegS-RseA system. Residues involved in the DegS allosteric switch are conserved in the DegP/HtrA and HtrA2/Omi families, suggesting that many PDZ proteases use a common mechanism of allosteric activation.
+The line below this paragraph, {{ABSTRACT_PUBMED_17981123}}, adds the Publication Abstract to the page
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 ==About this Structure==
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 [[Category: Periplasmic stress sensor]]
 [[Category: Protease]]
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