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| {{STRUCTURE_1yk9| PDB=1yk9 | SCENE= }} | | {{STRUCTURE_1yk9| PDB=1yk9 | SCENE= }} |
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| '''Crystal structure of a mutant form of the mycobacterial adenylyl cyclase Rv1625c'''
| | ===Crystal structure of a mutant form of the mycobacterial adenylyl cyclase Rv1625c=== |
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| ==Overview==
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| The Rv1625c Class III adenylyl cyclase from Mycobacterium tuberculosis is a homodimeric enzyme with two catalytic centers at the dimer interface, and shows sequence similarity with the mammalian adenylyl and guanylyl cyclases. Mutation of the substrate-specifying residues in the catalytic domain of Rv1625c, either independently or together, to those present in guanylyl cyclases not only failed to confer guanylyl cyclase activity to the protein, but also severely abrogated the adenylyl cyclase activity of the enzyme. Biochemical analysis revealed alterations in the behavior of the mutants on ion-exchange chromatography, indicating differences in the surface-exposed charge upon mutation of substrate-specifying residues. The mutant proteins showed alterations in oligomeric status as compared to the wild-type enzyme, and differing abilities to heterodimerize with the wild-type protein. The crystal structure of a mutant has been solved to a resolution of 2.7A. On the basis of the structure, and additional biochemical studies, we provide possible reasons for the altered properties of the mutant proteins, as well as highlight unique structural features of the Rv1625c adenylyl cyclase. | | The line below this paragraph, {{ABSTRACT_PUBMED_16403515}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16403515 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16403515}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Tb structural genomics consortium]] | | [[Category: Tb structural genomics consortium]] |
| [[Category: Tbsgc]] | | [[Category: Tbsgc]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 22:17:33 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:36:38 2008'' |