1rxm: Difference between revisions

Revision as of 09:34, 28 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1rxm.png

Template:STRUCTURE 1rxm

C-terminal region of FEN-1 bound to A. fulgidus PCNAC-terminal region of FEN-1 bound to A. fulgidus PCNA

Template:ABSTRACT PUBMED 14718165

About this StructureAbout this Structure

1RXM is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair., Chapados BR, Hosfield DJ, Han S, Qiu J, Yelent B, Shen B, Tainer JA, Cell. 2004 Jan 9;116(1):39-50. PMID:14718165

Page seeded by OCA on Mon Jul 28 09:34:38 2008

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OCA

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-'''C-terminal region of FEN-1 bound to A. fulgidus PCNA'''
+===C-terminal region of FEN-1 bound to A. fulgidus PCNA===
-==Overview==
+<!--
-Flap EndoNuclease-1 (FEN-1) and the processivity factor proliferating cell nuclear antigen (PCNA) are central to DNA replication and repair. To clarify the molecular basis of FEN-1 specificity and PCNA activation, we report here structures of FEN-1:DNA and PCNA:FEN-1-peptide complexes, along with fluorescence resonance energy transfer (FRET) and mutational results. FEN-1 binds the unpaired 3' DNA end (3' flap), opens and kinks the DNA, and promotes conformational closing of a flexible helical clamp to facilitate 5' cleavage specificity. Ordering of unstructured C-terminal regions in FEN-1 and PCNA creates an intermolecular beta sheet interface that directly links adjacent PCNA and DNA binding regions of FEN-1 and suggests how PCNA stimulates FEN-1 activity. The DNA and protein conformational changes, composite complex structures, FRET, and mutational results support enzyme-PCNA alignments and a kinked DNA pivot point that appear suitable to coordinate rotary handoffs of kinked DNA intermediates among enzymes localized by the three PCNA binding sites.
+The line below this paragraph, {{ABSTRACT_PUBMED_14718165}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 14718165 is the PubMed ID number.
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+{{ABSTRACT_PUBMED_14718165}}
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 [[Category: Sliding clamp]]
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