3brg: Difference between revisions

Revision as of 08:59, 28 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:3brg.png

Template:STRUCTURE 3brg

CSL (RBP-Jk) bound to DNACSL (RBP-Jk) bound to DNA

Template:ABSTRACT PUBMED 18381292

About this StructureAbout this Structure

3BRG is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

RAM induced allostery facilitates assembly of a notch pathway active transcription complex., Friedmann DR, Wilson JJ, Kovall RA, J Biol Chem. 2008 Apr 1;. PMID:18381292

Page seeded by OCA on Mon Jul 28 08:59:23 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-'''CSL (RBP-Jk) bound to DNA'''
+===CSL (RBP-Jk) bound to DNA===
-==Overview==
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-The Notch pathway is a conserved cell-to-cell signaling mechanism, in which extracellular signals are transduced into transcriptional outputs through the nuclear effector CSL. CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. The RAM domain of NotchIC avidly interacts with CSL; however, its role in assembly of the CSL-NotchIC-Mastermind ternary complex is not understood. Here we provide a comprehensive thermodynamic, structural, and biochemical analysis of the RAM-CSL interaction for components from both mouse and worm. Our binding data shows that RAM and CSL form a high affinity complex in the presence or absence of DNA. Our structural studies reveal a striking distal conformational change in CSL upon RAM binding, which creates a docking site for Mastermind to bind to the complex. Finally, we show that the addition of a RAM peptide in trans facilitates formation of the CSL-NotchIC-Mastermind ternary complex in vitro.
+The line below this paragraph, {{ABSTRACT_PUBMED_18381292}}, adds the Publication Abstract to the page
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+{{ABSTRACT_PUBMED_18381292}}
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 [[Category: Transcription]]
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