1txe: Difference between revisions

Revision as of 07:24, 28 July 2008

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File:1txe.png

Template:STRUCTURE 1txe

Solution structure of the active-centre mutant Ile14Ala of the histidine-containing phosphocarrier protein (HPr) from Staphylococcus carnosusSolution structure of the active-centre mutant Ile14Ala of the histidine-containing phosphocarrier protein (HPr) from Staphylococcus carnosus

Template:ABSTRACT PUBMED 15606769

About this StructureAbout this Structure

1TXE is a Single protein structure of sequence from Staphylococcus carnosus. Full experimental information is available from OCA.

ReferenceReference

Solution structure of the active-centre mutant I14A of the histidine-containing phosphocarrier protein from Staphylococcus carnosus., Moglich A, Koch B, Gronwald W, Hengstenberg W, Brunner E, Kalbitzer HR, Eur J Biochem. 2004 Dec;271(23-24):4815-24. PMID:15606769

15N and 1H NMR study of histidine containing protein (HPr) from Staphylococcus carnosus at high pressure., Kalbitzer HR, Gorler A, Li H, Dubovskii PV, Hengstenberg W, Kowolik C, Yamada H, Akasaka K, Protein Sci. 2000 Apr;9(4):693-703. PMID:10794411

NMR-spectroscopic mapping of an engineered cavity in the I14A mutant of HPr from Staphylococcus carnosus using xenon., Groger C, Moglich A, Pons M, Koch B, Hengstenberg W, Kalbitzer HR, Brunner E, J Am Chem Soc. 2003 Jul 23;125(29):8726-7. PMID:12862458

DRESS: a database of REfined solution NMR structures., Nabuurs SB, Nederveen AJ, Vranken W, Doreleijers JF, Bonvin AM, Vuister GW, Vriend G, Spronk CA, Proteins. 2004 May 15;55(3):483-6. PMID:15103611

Refinement of protein structures in explicit solvent., Linge JP, Williams MA, Spronk CA, Bonvin AM, Nilges M, Proteins. 2003 Feb 15;50(3):496-506. PMID:12557191

Page seeded by OCA on Mon Jul 28 07:24:56 2008

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-[[Image:1txe.gif|left|200px]]
+{{Seed}}
+[[Image:1txe.png|left|200px]]
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 {{STRUCTURE_1txe|  PDB=1txe  |  SCENE=  }}
-'''Solution structure of the active-centre mutant Ile14Ala of the histidine-containing phosphocarrier protein (HPr) from Staphylococcus carnosus'''
+===Solution structure of the active-centre mutant Ile14Ala of the histidine-containing phosphocarrier protein (HPr) from Staphylococcus carnosus===
-==Overview==
+<!--
-High-pressure NMR experiments performed on the histidine-containing phosphocarrier protein (HPr) from Staphylococcus carnosus have shown that residue Ile14, which is located in the active-centre loop, exhibits a peculiarly small pressure response. In contrast, the rest of the loop shows strong pressure effects as is expected for typical protein interaction sites. To elucidate the structural role of this residue, the mutant protein HPr(I14A), in which Ile14 is replaced by Ala, was produced and studied by solution NMR spectroscopy. On the basis of 1406 structural restraints including 20 directly detected hydrogen bonds, 49 1H(N)-15N, and 25 1H(N)-1Halpha residual dipolar couplings, a well resolved three-dimensional structure could be determined. The overall fold of the protein is not influenced by the mutation but characteristic conformational changes are introduced into the active-centre loop. They lead to a displacement of the ring system of His15 and a distortion of the N-terminus of the first helix, which supports the histidine ring. In addition, the C-terminal helix is bent because the side chain of Leu86 located at the end of this helix partly fills the hydrophobic cavity created by the mutation. Xenon, which is known to occupy hydrophobic cavities, causes a partial reversal of the mutation-induced structural effects. The observed structural changes explain the reduced phosphocarrier activity of the mutant and agree well with the earlier suggestion that Ile14 represents an anchoring point stabilizing the active-centre loop in its correct conformation.
+The line below this paragraph, {{ABSTRACT_PUBMED_15606769}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 15606769 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_15606769}}
 ==About this Structure==
-TXE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_carnosus Staphylococcus carnosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXE OCA].
+TXE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_carnosus Staphylococcus carnosus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXE OCA].
 ==Reference==
 Solution structure of the active-centre mutant I14A of the histidine-containing phosphocarrier protein from Staphylococcus carnosus., Moglich A, Koch B, Gronwald W, Hengstenberg W, Brunner E, Kalbitzer HR, Eur J Biochem. 2004 Dec;271(23-24):4815-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15606769 15606769]
+N and 1H NMR study of histidine containing protein (HPr) from Staphylococcus carnosus at high pressure., Kalbitzer HR, Gorler A, Li H, Dubovskii PV, Hengstenberg W, Kowolik C, Yamada H, Akasaka K, Protein Sci. 2000 Apr;9(4):693-703. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10794411 10794411]
+NMR-spectroscopic mapping of an engineered cavity in the I14A mutant of HPr from Staphylococcus carnosus using xenon., Groger C, Moglich A, Pons M, Koch B, Hengstenberg W, Kalbitzer HR, Brunner E, J Am Chem Soc. 2003 Jul 23;125(29):8726-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12862458 12862458]
+DRESS: a database of REfined solution NMR structures., Nabuurs SB, Nederveen AJ, Vranken W, Doreleijers JF, Bonvin AM, Vuister GW, Vriend G, Spronk CA, Proteins. 2004 May 15;55(3):483-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15103611 15103611]
+Refinement of protein structures in explicit solvent., Linge JP, Williams MA, Spronk CA, Bonvin AM, Nilges M, Proteins. 2003 Feb 15;50(3):496-506. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12557191 12557191]
 [[Category: Single protein]]
 [[Category: Staphylococcus carnosus]]
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 [[Category: Structural genomic]]
 [[Category: Structural proteomics in europe]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:12:15 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:24:56 2008''