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| {{STRUCTURE_2ohy| PDB=2ohy | SCENE= }} | | {{STRUCTURE_2ohy| PDB=2ohy | SCENE= }} |
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| '''X-ray Crystal Structure of Tyrosine Aminomutase from streptomyces globisporus'''
| | ===X-ray Crystal Structure of Tyrosine Aminomutase from streptomyces globisporus=== |
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| ==Overview==
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| The SgcC4 l-tyrosine 2,3-aminomutase (SgTAM) catalyzes the formation of (S)-beta-tyrosine in the biosynthetic pathway of the enediyne antitumor antibiotic C-1027. SgTAM is homologous to the histidine ammonia lyase family of enzymes whose activity is dependent on the methylideneimidazole-5-one (MIO) cofactor. Unlike the lyase enzymes, SgTAM catalyzes additional chemical transformations resulting in an overall stereospecific 1,2-amino shift in the substrate l-tyrosine to generate (S)-beta-tyrosine. Previously, we provided kinetic, spectroscopic, and mutagenesis data supporting the presence of MIO in the active site of SgTAM [Christenson, S. D.; Wu, W.; Spies, A.; Shen, B.; and Toney, M. D. (2003) Biochemistry 42, 12708-12718]. Here we report the first X-ray crystal structure of an MIO-containing aminomutase, SgTAM, and confirm the structural homology of SgTAM to ammonia lyases. Comparison of the structure of SgTAM to the l-tyrosine ammonia lyase from Rhodobacter sphaeroides provides insight into the structural basis for aminomutase activity. The results show that SgTAM has a closed active site well suited to retain ammonia and minimize the formation of lyase elimination products. The amino acid determinants for substrate recognition and catalysis can be predicted from the structure, setting the framework for detailed mechanistic investigations. | | The line below this paragraph, {{ABSTRACT_PUBMED_17516659}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17516659 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17516659}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: 4-methylideneimidizole-5-one]] | | [[Category: 4-methylideneimidizole-5-one]] |
| [[Category: Mio]] | | [[Category: Mio]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:57:48 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:08:16 2008'' |