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| {{STRUCTURE_2frp| PDB=2frp | SCENE= }} | | {{STRUCTURE_2frp| PDB=2frp | SCENE= }} |
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| '''Bacteriophage HK97 Expansion Intermediate IV'''
| | ===Bacteriophage HK97 Expansion Intermediate IV=== |
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| ==Overview==
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| Maturation of the bacteriophage HK97 capsid from a precursor (Prohead II) to the mature state (Head II) involves a 60 A radial expansion. The mature particle is formed by 420 copies of the major capsid protein organized on a T = 7 laevo lattice with each subunit covalently crosslinked to two neighbors. Well-characterized pH 4 expansion intermediates make HK97 valuable for investigating quaternary structural dynamics. Here, we use X-ray crystallography and cryo-EM to demonstrate that in the final transition in maturation (requiring neutral pH), pentons in Expansion Intermediate IV (EI-IV) reversibly sample 14 A translations and 6 degrees rotations relative to a fixed hexon lattice. The limit of this trajectory corresponds to the Head II conformation that is secured at this extent only by the formation of the final class of covalent crosslinks. Mutants that cannot crosslink or EI-IV particles that have been rendered incapable of forming the final crosslink remain in the EI-IV state.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17098191}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17098191 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17098191}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Expansion intermediate]] | | [[Category: Expansion intermediate]] |
| [[Category: Hk97]] | | [[Category: Hk97]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:14:16 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:13:11 2008'' |