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| {{STRUCTURE_2ezp| PDB=2ezp | SCENE= }} | | {{STRUCTURE_2ezp| PDB=2ezp | SCENE= }} |
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| '''SOLUTION NMR STRUCTURE OF ECTODOMAIN OF SIV GP41, MODELS 1-10 OF AN ENSEMBLE OF 40 SIMULATED ANNEALING STRUCTURES'''
| | ===SOLUTION NMR STRUCTURE OF ECTODOMAIN OF SIV GP41, MODELS 1-10 OF AN ENSEMBLE OF 40 SIMULATED ANNEALING STRUCTURES=== |
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| ==Overview==
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| The solution structure of the ectodomain of simian immunodeficiency virus (SIV) gp41 (e-gp41), consisting of residues 27-149, has been determined by multidimensional heteronuclear NMR spectroscopy. SIV e-gp41 is a symmetric 44 kDa trimer with each subunit consisting of antiparallel N-terminal (residues 30-80) and C-terminal (residues 107-147) helices connected by a 26 residue loop (residues 81-106). The N-terminal helices of each subunit form a parallel coiled-coil structure in the interior of the complex which is surrounded by the C-terminal helices located on the exterior of the complex. The loop region is ordered and displays numerous intermolecular and non-sequential intramolecular contacts. The helical core of SIV e-gp41 is similar to recent X-ray structures of truncated constructs of the helical core of HIV-1 e-gp41. The present structure establishes unambiguously the connectivity of the N- and C-terminal helices in the trimer, and characterizes the conformation of the intervening loop, which has been implicated by mutagenesis and antibody epitope mapping to play a key role in gp120 association. In conjunction with previous studies, the solution structure of the SIV e-gp41 ectodomain provides insight into the binding site of gp120 and the mechanism of cell fusion. The present structure of SIV e-gp41 represents one of the largest protein structures determined by NMR to date. | | The line below this paragraph, {{ABSTRACT_PUBMED_9707417}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 9707417 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_9707417}} |
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| ==About this Structure== | | ==About this Structure== |
| 2EZP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Simian_immunodeficiency_virus Simian immunodeficiency virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EZP OCA]. | | 2EZP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Simian_immunodeficiency_virus Simian immunodeficiency virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EZP OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Siv gp41 ectodomain]] | | [[Category: Siv gp41 ectodomain]] |
| [[Category: Virus envelope protein]] | | [[Category: Virus envelope protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:17:35 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:52:08 2008'' |