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| [[Image:2b3r.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2b3r| PDB=2b3r | SCENE= }} | | {{STRUCTURE_2b3r| PDB=2b3r | SCENE= }} |
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| '''Crystal structure of the C2 domain of class II phosphatidylinositide 3-kinase C2'''
| | ===Crystal structure of the C2 domain of class II phosphatidylinositide 3-kinase C2=== |
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| ==Overview==
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| Phosphatidylinositide (PtdIns) 3-kinase catalyzes the addition of a phosphate group to the 3'-position of phosphatidyl inositol. Accumulated evidence shows that PtdIns 3-kinase can provide a critical signal for cell proliferation, cell survival, membrane trafficking, glucose transport, and membrane ruffling. Mammalian PtdIns 3-kinases are divided into three classes based on structure and substrate specificity. A unique characteristic of class II PtdIns 3-kinases is the presence of both a phox homolog domain and a C2 domain at the C terminus. The biological function of the C2 domain of the class II PtdIns 3-kinases remains to be determined. We have determined the crystal structure of the mCPK-C2 domain, which is the first three-dimensional structural model of a C2 domain of class II PtdIns 3-kinases. Structural studies reveal that the mCPK-C2 domain has a typical anti-parallel beta-sandwich fold. Scrutiny of the surface of this C2 domain has identified three small, shallow sulfate-binding sites. On the basis of the structural features of these sulfate-binding sites, we have studied the lipid binding properties of the mCPK-C2 domain by site-directed mutagenesis. Our results show that this C2 domain binds specifically to PtdIns(3,4)P(2) and PtdIns(4,5)P(2) and that three lysine residues at SBS I site, Lys-1420, Lys-1432, and Lys-1434, are responsible for the phospholipid binding affinity.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16338929}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16338929 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16338929}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Lipid binding]] | | [[Category: Lipid binding]] |
| [[Category: Pi3-kinase]] | | [[Category: Pi3-kinase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:49:04 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:12:55 2008'' |