1n4f: Difference between revisions

Revision as of 22:58, 27 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1n4f.png

Template:STRUCTURE 1n4f

Para-Arsanilate Derivative of Hen Egg-White LysozymePara-Arsanilate Derivative of Hen Egg-White Lysozyme

Template:ABSTRACT PUBMED 12777806

About this StructureAbout this Structure

1N4F is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

ReferenceReference

Phasing power at the K absorption edge of organic arsenic., Retailleau P, Prange T, Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):887-96. Epub 2003, Apr 25. PMID:12777806

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OCA

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-'''Para-Arsanilate Derivative of Hen Egg-White Lysozyme'''
+===Para-Arsanilate Derivative of Hen Egg-White Lysozyme===
-==Overview==
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-Single/multiple-wavelength anomalous dispersion (SAD/MAD) experiments were performed on a crystal of an organic arsenic derivative of hen egg-white lysozyme. A para-arsanilate compound used as a crystallizing reagent was incorporated into the ordered solvent region of the lysozyme molecule. Diffraction data were collected to high resolution (&lt;/=2.0 A) at three wavelengths around the K edge (1.04 A) of arsenic at beamline BM30A, ESRF synchrotron. Anomalous Patterson maps clearly showed the main arsanilate site to be between three symmetry-related lysozyme molecules, at a location previously occupied by a para-toluenesulfonate anion. MAD phases at 2 A derived using the program SHARP led to an electron-density map of sufficient quality to start manual building of the protein model. Amplitudes from a second crystal measured to a resolution of 1.8 A at the peak wavelength revealed two additional heavy-atom sites, which reinforced the anomalous subset model and therefore dramatically improved the phasing power of the arsenic derivative. The subsequent solvent-flattened map was of such high accuracy that the program ARP/wARP was able to build a nearly complete model automatically. This work emphasizes the great potential of arsenic for de novo structure determination using anomalous dispersion methods.
+The line below this paragraph, {{ABSTRACT_PUBMED_12777806}}, adds the Publication Abstract to the page
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+{{ABSTRACT_PUBMED_12777806}}
 ==About this Structure==
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 [[Category: Retailleau, P.]]
 [[Category: Hydrolase]]
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