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| [[Image:1suu.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1suu| PDB=1suu | SCENE= }} | | {{STRUCTURE_1suu| PDB=1suu | SCENE= }} |
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| '''Structure of DNA gyrase A C-terminal domain'''
| | ===Structure of DNA gyrase A C-terminal domain=== |
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| ==Overview==
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| DNA gyrase is unique among enzymes for its ability to actively introduce negative supercoils into DNA. This function is mediated in part by the C-terminal domain of its A subunit (GyrA CTD). Here, we report the crystal structure of this approximately 35-kDa domain determined to 1.75-A resolution. The GyrA CTD unexpectedly adopts an unusual fold, which we term a beta-pinwheel, that is globally reminiscent of a beta-propeller but is built of blades with a previously unobserved topology. A large, conserved basic patch on the outer edge of this domain suggests a likely site for binding and bending DNA; fluorescence resonance energy transfer-based assays show that the GyrA CTD is capable of bending DNA by > or =180 degrees over a 40-bp region. Surprisingly, we find that the CTD of the topoisomerase IV A subunit, which shares limited sequence homology with the GyrA CTD, also bends DNA. Together, these data provide a physical explanation for the ability of DNA gyrase to constrain a positive superhelical DNA wrap, and also suggest that the particular substrate preferences of topoisomerase IV might be dictated in part by the function of this domain.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15123801}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15123801 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15123801}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Beta-propeller]] | | [[Category: Beta-propeller]] |
| [[Category: Topoisomerase,dna gyrase]] | | [[Category: Topoisomerase,dna gyrase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:09:34 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:42:00 2008'' |