2dhd: Difference between revisions

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-[[Image:2dhd.gif|left|200px]]
+{{Seed}}
+[[Image:2dhd.png|left|200px]]
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 {{STRUCTURE_2dhd|  PDB=2dhd  |  SCENE=  }}
-'''CRYSTALLOGRAPHIC ANALYSIS OF THE CATALYTIC MECHANISM OF HALOALKANE DEHALOGENASE'''
+===CRYSTALLOGRAPHIC ANALYSIS OF THE CATALYTIC MECHANISM OF HALOALKANE DEHALOGENASE===
-==Overview==
+<!--
-Crystal structures of haloalkane dehalogenase were determined in the presence of the substrate 1,2-dichloroethane. At pH 5 and 4 degrees C, substrate is bound in the active site without being converted; warming to room temperature causes the substrate's carbon-chlorine bond to be broken, producing a chloride ion with concomitant alkylation of the active-site residue Asp124. At pH 6 and room temperature the alkylated enzyme is hydrolysed by a water molecule activated by the His289-Asp260 pair in the active site. These results show that catalysis by the dehalogenase proceeds by a two-step mechanism involving an ester intermediate covalently bound at Asp124.
+The line below this paragraph, {{ABSTRACT_PUBMED_8515812}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 8515812 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_8515812}}
 ==About this Structure==
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 [[Category: Verschueren, K H.G.]]
 [[Category: Dehalogenase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 00:25:58 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:00:18 2008''