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| [[Image:1suc.jpg|left|200px]] | | {{Seed}} |
| | [[Image:1suc.png|left|200px]] |
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| {{STRUCTURE_1suc| PDB=1suc | SCENE= }} | | {{STRUCTURE_1suc| PDB=1suc | SCENE= }} |
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| '''CALCIUM-INDEPENDENT SUBTILISIN BY DESIGN'''
| | ===CALCIUM-INDEPENDENT SUBTILISIN BY DESIGN=== |
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| ==Overview==
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| A version of subtilisin BPN' lacking the high affinity calcium site (site A) has been produced through genetic engineering methods, and its crystal structure refined at 1.8 A resolution. This protein and the corresponding version containing the calcium A site are described and compared. The deletion of residues 75-83 was made in the context of four site-specific replacements previously shown to stabilize subtilisin. The helix that in wild type is interrupted by the calcium binding loop, is continuous in the deletion mutant, with normal geometry. A few residues adjacent to the loop, principally those that were involved in calcium coordination, are repositioned and/or destabilized by the deletion. Because refolding is greatly facilitated by the absence of the Ca-loop, this protein offers a new vehicle for analysis and dissection of the folding reaction. This is among the largest internal changes to a protein to be described at atomic resolution.
| | The line below this paragraph, {{ABSTRACT_PUBMED_8332608}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 8332608 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_8332608}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Gallagher, T.]] | | [[Category: Gallagher, T.]] |
| [[Category: Gilliland, G L.]] | | [[Category: Gilliland, G L.]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:08:41 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:37:08 2008'' |