2c83: Difference between revisions

← Older edit Newer edit →

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2c83.gif|left|200px]]
+{{Seed}}
+[[Image:2c83.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_2c83|  PDB=2c83  |  SCENE=  }}
-'''CRYSTAL STRUCTURE OF THE SIALYLTRANSFERASE PM0188'''
+===CRYSTAL STRUCTURE OF THE SIALYLTRANSFERASE PM0188===
-==Overview==
+<!--
-PM0188 is a newly identified sialyltransferase from P. multocida which transfers sialic acid from cytidine 5'-monophosphonuraminic acid (CMP-NeuAc) to an acceptor sugar. Although sialyltransferases are involved in important biological functions like cell-cell recognition, cell differentiation and receptor-ligand interactions, little is known about their catalytic mechanism. Here, we report the X-ray crystal structures of PM0188 in the presence of an acceptor sugar and a donor sugar analogue, revealing the precise mechanism of sialic acid transfer. Site-directed mutagenesis, kinetic assays, and structural analysis show that Asp141, His311, Glu338, Ser355 and Ser356 are important catalytic residues; Asp141 is especially crucial as it acts as a general base. These complex structures provide insights into the mechanism of sialyltransferases and the structure-based design of specific inhibitors.
+The line below this paragraph, {{ABSTRACT_PUBMED_18304450}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 18304450 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_18304450}}
 ==About this Structure==
@@ Line 20: / Line 24: @@
 ==Reference==
 Structural analysis of sialyltransferase PM0188 from Pasteurella multocida complexed with donor analogue and acceptor sugar., Kim DU, Yoo JH, Lee YJ, Kim KS, Cho HS, BMB Rep. 2008 Jan 31;41(1):48-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18304450 18304450]
+Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog., Chiu CP, Watts AG, Lairson LL, Gilbert M, Lim D, Wakarchuk WW, Withers SG, Strynadka NC, Nat Struct Mol Biol. 2004 Feb;11(2):163-70. Epub 2004 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14730352 14730352]
+Crystallization and preliminary X-ray crystallographic analysis of the alpha-2,6-sialyltransferase PM0188 from Pasteurella multosida., Kim DU, Yoo JH, Ryu K, Cho HS, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Feb 1;62(Pt, 2):142-4. Epub 2006 Jan 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511286 16511286]
 [[Category: Pasteurella multocida]]
 [[Category: Single protein]]
@@ Line 28: / Line 36: @@
 [[Category: Sialyltransferase]]
 [[Category: Transferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:25:34 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:21:57 2008''