2b4z: Difference between revisions

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{{STRUCTURE_2b4z|  PDB=2b4z  |  SCENE=  }}  
{{STRUCTURE_2b4z|  PDB=2b4z  |  SCENE=  }}  


'''Crystal structure of cytochrome C from bovine heart at 1.5 A resolution.'''
===Crystal structure of cytochrome C from bovine heart at 1.5 A resolution.===




==Overview==
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Cytochrome c is one of the most studied proteins probably due to its electron-transfer properties in aerobic and anaerobic respiration. Particularly, cytochrome c from bovine heart is a small protein, M(r) 12,230 Da, globular (hydrodynamic diameter of 3.4 nm), soluble in different buffer solutions, and commercially available. Despite being a quite well-studied protein and relatively easy to manipulate from the biochemical and electrochemical viewpoint, its 3D structure has never been published. In this work, the purification, crystallization and 3D structure of one of the cytochrome c isoforms is presented to 1.5 A resolution. It is also shown how the presence of isoforms made both the purification and crystallization steps difficult. Finally, a new approach for protein electrocrystallization and design of biosensors is presented.
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==About this Structure==
==About this Structure==
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[[Category: Ferric form]]
[[Category: Ferric form]]
[[Category: Low ionic strength]]
[[Category: Low ionic strength]]
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