|
|
| Line 1: |
Line 1: |
| [[Image:1x9z.jpg|left|200px]] | | {{Seed}} |
| | [[Image:1x9z.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1x9z| PDB=1x9z | SCENE= }} | | {{STRUCTURE_1x9z| PDB=1x9z | SCENE= }} |
|
| |
|
| '''Crystal structure of the MutL C-terminal domain'''
| | ===Crystal structure of the MutL C-terminal domain=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| MutL assists the mismatch recognition protein MutS to initiate and coordinate mismatch repair in species ranging from bacteria to humans. The MutL N-terminal ATPase domain is highly conserved, but the C-terminal region shares little sequence similarity among MutL homologs. We report here the crystal structure of the Escherichia coli MutL C-terminal dimerization domain and the likelihood of its conservation among MutL homologs. A 100-residue proline-rich linker between the ATPase and dimerization domains, which generates a large central cavity in MutL dimers, tolerates sequence substitutions and deletions of one-third of its length with no functional consequences in vivo or in vitro. Along the surface of the central cavity, residues essential for DNA binding are located in both the N- and C-terminal domains. Each domain of MutL interacts with UvrD helicase and is required for activating the helicase activity. The DNA-binding capacity of MutL is correlated with the level of UvrD activation. A model of how MutL utilizes its ATPase and DNA-binding activities to mediate mismatch-dependent activation of MutH endonuclease and UvrD helicase is proposed.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15470502}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15470502 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_15470502}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 31: |
Line 35: |
| [[Category: Alpha-beta fold]] | | [[Category: Alpha-beta fold]] |
| [[Category: Dimer]] | | [[Category: Dimer]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:45:50 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:39:58 2008'' |