|
|
| Line 1: |
Line 1: |
| [[Image:2b4h.gif|left|200px]] | | {{Seed}} |
| | [[Image:2b4h.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_2b4h| PDB=2b4h | SCENE= }} | | {{STRUCTURE_2b4h| PDB=2b4h | SCENE= }} |
|
| |
|
| '''Crystal Structure of the Rhesus Rotavirus VP5 Antigen Domain Dimer'''
| | ===Crystal Structure of the Rhesus Rotavirus VP5 Antigen Domain Dimer=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| The spike protein VP4 is a key component of the membrane penetration apparatus of rotavirus, a nonenveloped virus that causes childhood gastroenteritis. Trypsin cleavage of VP4 produces a fragment, VP5*, with a potential membrane interaction region, and primes rotavirus for cell entry. During entry, the part of VP5* that protrudes from the virus folds back on itself and reorganizes from a local dimer to a trimer. Here, we report that a globular domain of VP5*, the VP5* antigen domain, is an autonomously folding unit that alternatively forms well-ordered dimers and trimers. Because the domain contains heterotypic neutralizing epitopes and is soluble when expressed directly, it is a promising potential subunit vaccine component. X-ray crystal structures show that the dimer resembles the spike body on trypsin-primed virions, and the trimer resembles the folded-back form of the spike. The same structural elements pack differently to form key intermolecular contacts in both oligomers. The intrinsic molecular property of alternatively forming dimers and trimers facilitates the VP5* reorganization, which is thought to mediate membrane penetration during cell entry. | | The line below this paragraph, {{ABSTRACT_PUBMED_16511559}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16511559 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_16511559}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 30: |
Line 34: |
| [[Category: Rearrangement]] | | [[Category: Rearrangement]] |
| [[Category: Spike protein]] | | [[Category: Spike protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:50:40 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:49:27 2008'' |