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| {{STRUCTURE_1yx9| PDB=1yx9 | SCENE= }} | | {{STRUCTURE_1yx9| PDB=1yx9 | SCENE= }} |
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| '''Effect of Dimethyl Sulphoxide on the crystal structure of Porcine Pepsin'''
| | ===Effect of Dimethyl Sulphoxide on the crystal structure of Porcine Pepsin=== |
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| ==Overview==
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| The structure of porcine pepsin crystallized in the presence of dimethyl sulphoxide has been analysed by X-ray crystallography to obtain insights into the structural events that occur at the onset of chemical denaturation of proteins. The results show that one dimethyl sulphoxide molecule occupies a site on the surface of pepsin interacting with two of its residues. An increase in the average temperature factor of pepsin in the presence of dimethyl sulphoxide has been observed indicating protein destabilization induced by the denaturant. Significant increase in the temperature factor and weakening of the electron density have been observed for the catalytic water molecule located between the active aspartates. The conformation of pepsin remains unchanged in the crystal structure. However, the enzyme assay and circular dichroism studies indicate that dimethyl sulphoxide causes a slight change in the secondary structure and complete loss of activity of pepsin in solution. | | The line below this paragraph, {{ABSTRACT_PUBMED_15883044}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15883044 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15883044}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Pepsin]] | | [[Category: Pepsin]] |
| [[Category: Protein denaturation]] | | [[Category: Protein denaturation]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:54:56 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:06:58 2008'' |