2d8p: Difference between revisions

Revision as of 12:49, 27 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:2d8p.png

Template:STRUCTURE 2d8p

Structure of HYPER-VIL-thaumatinStructure of HYPER-VIL-thaumatin

Template:ABSTRACT PUBMED 16510975

About this StructureAbout this Structure

2D8P is a Single protein structure of sequence from Thaumatococcus daniellii. Full crystallographic information is available from OCA.

ReferenceReference

New methods to prepare iodinated derivatives by vaporizing iodine labelling (VIL) and hydrogen peroxide VIL (HYPER-VIL)., Miyatake H, Hasegawa T, Yamano A, Acta Crystallogr D Biol Crystallogr. 2006 Mar;62(Pt 3):280-9. Epub 2006, Feb 22. PMID:16510975

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-'''Structure of HYPER-VIL-thaumatin'''
+===Structure of HYPER-VIL-thaumatin===
-==Overview==
+<!--
-New techniques are presented for the preparation of iodine derivatives, involving vapour diffusion of iodine. Firstly, in the vaporizing iodine labelling (VIL) technique, a small amount of KI/I(2) solution is enclosed in a crystallization well, with the result that gaseous I(2) molecules diffuse into the crystallization droplets without exerting substantial changes in ionic strength in the target crystals. Once they have diffused into the droplet, the I(2) molecules sometimes iodinate accessible tyrosines at ortho positions. Secondly, when iodination is insufficient, the hydrogen peroxide VIL (HYPER-VIL) technique can be further applied to increase the iodination ratio by the addition of a small droplet of hydrogen peroxide (H(2)O(2)) to the crystallization well; the gaseous H(2)O(2) also diffuses into the crystallization droplet to emphasize the iodination. These techniques are most effective for phase determination when coupled with softer X-rays, such as those from Cu Kalpha or Cr Kalpha radiation. The effectiveness of these techniques was assessed using five different crystals. Four of the crystals were successfully iodinated, providing sufficient phasing power for structure determination.
+The line below this paragraph, {{ABSTRACT_PUBMED_16510975}}, adds the Publication Abstract to the page
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 [[Category: Yamano, A.]]
 [[Category: Iodo-tyrosine]]
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