2i3q: Difference between revisions

Revision as of 12:06, 6 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:2i3q.png

Template:STRUCTURE 2i3q

Q44V mutant of Homing Endonuclease I-CreIQ44V mutant of Homing Endonuclease I-CreI

Template:ABSTRACT PUBMED 16971456

About this StructureAbout this Structure

2I3Q is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.

ReferenceReference

Homing endonuclease I-CreI derivatives with novel DNA target specificities., Rosen LE, Morrison HA, Masri S, Brown MJ, Springstubb B, Sussman D, Stoddard BL, Seligman LM, Nucleic Acids Res. 2006;34(17):4791-800. Epub 2006 Sep 13. PMID:16971456

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OCA

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-'''Q44V mutant of Homing Endonuclease I-CreI'''
+===Q44V mutant of Homing Endonuclease I-CreI===
-==Overview==
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-Homing endonucleases are highly specific enzymes, capable of recognizing and cleaving unique DNA sequences in complex genomes. Since such DNA cleavage events can result in targeted allele-inactivation and/or allele-replacement in vivo, the ability to engineer homing endonucleases matched to specific DNA sequences of interest would enable powerful and precise genome manipulations. We have taken a step-wise genetic approach in analyzing individual homing endonuclease I-CreI protein/DNA contacts, and describe here novel interactions at four distinct target site positions. Crystal structures of two mutant endonucleases reveal the molecular interactions responsible for their altered DNA target specificities. We also combine novel contacts to create an endonuclease with the predicted target specificity. These studies provide important insights into engineering homing endonucleases with novel target specificities, as well as into the evolution of DNA recognition by this fascinating family of proteins.
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 ==About this Structure==
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 [[Category: Homing endonuclease]]
 [[Category: I-crei]]
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