4uag: Difference between revisions

Revision as of 13:53, 3 July 2008

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File:4uag.png

Template:STRUCTURE 4uag

UDP-N-ACETYLMURAMOYL-L-ALANINE:D-GLUTAMATE LIGASEUDP-N-ACETYLMURAMOYL-L-ALANINE:D-GLUTAMATE LIGASE

Template:ABSTRACT PUBMED 10356330

About this StructureAbout this Structure

4UAG is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Determination of the MurD mechanism through crystallographic analysis of enzyme complexes., Bertrand JA, Auger G, Martin L, Fanchon E, Blanot D, Le Beller D, van Heijenoort J, Dideberg O, J Mol Biol. 1999 Jun 11;289(3):579-90. PMID:10356330

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OCA

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-[[Image:4uag.jpg|left|200px]]
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-'''UDP-N-ACETYLMURAMOYL-L-ALANINE:D-GLUTAMATE LIGASE'''
+===UDP-N-ACETYLMURAMOYL-L-ALANINE:D-GLUTAMATE LIGASE===
-==Overview==
+<!--
-UDP -N- acetylmuramoyl- L -alanine: D -glutamate (MurD) ligase catalyses the addition of d -glutamate to the nucleotide precursor UDP -N- acetylmuramoyl- L -alanine (UMA). The crystal structures of three complexes of Escherichia coli MurD with a variety of substrates and products have been determined to high resolution. These include (1) the quaternary complex of MurD, the substrate UMA, the product ADP, and Mg2+, (2) the quaternary complex of MurD, the substrate UMA, the product ADP, and Mn2+, and (3) the binary complex of MurD with the product UDP - N- acetylmuramoyl- L -alanine- D -glutamate (UMAG). The reaction mechanism supported by these structures proceeds by the phosphorylation of the C-terminal carboxylate group of UMA by the gamma-phosphate group of ATP to form an acyl-phosphate intermediate, followed by the nucleophilic attack by the amino group of D-glutamate to produce UMAG. A key feature in the reaction intermediate is the presence of two magnesium ions bridging negatively charged groups.
+The line below this paragraph, {{ABSTRACT_PUBMED_10356330}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 10356330 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_10356330}}
 ==About this Structure==
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 [[Category: Murd]]
 [[Category: Peptidoglycan synthesis]]
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