1m6i: Difference between revisions

Revision as of 23:19, 2 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1m6i.png

Template:STRUCTURE 1m6i

Crystal Structure of Apoptosis Inducing Factor (AIF)Crystal Structure of Apoptosis Inducing Factor (AIF)

Template:ABSTRACT PUBMED 12198487

About this StructureAbout this Structure

1M6I is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

DNA binding is required for the apoptogenic action of apoptosis inducing factor., Ye H, Cande C, Stephanou NC, Jiang S, Gurbuxani S, Larochette N, Daugas E, Garrido C, Kroemer G, Wu H, Nat Struct Biol. 2002 Sep;9(9):680-4. PMID:12198487

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-'''Crystal Structure of Apoptosis Inducing Factor (AIF)'''
+===Crystal Structure of Apoptosis Inducing Factor (AIF)===
-==Overview==
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-The execution of apoptosis or programmed cell death comprises both caspase-dependent and caspase-independent processes. Apoptosis inducing factor (AIF) was identified as a major player in caspase-independent cell death. It induces chromatin condensation and initial DNA cleavage via an unknown molecular mechanism. Here we report the crystal structure of human AIF at 1.8 A resolution. The structure reveals the presence of a strong positive electrostatic potential at the AIF surface, although the calculated isoelectric point for the entire protein is neutral. We show that recombinant AIF interacts with DNA in a sequence-independent manner. In addition, in cells treated with an apoptotic stimulus, endogenous AIF becomes co-localized with DNA at an early stage of nuclear morphological changes. Structure-based mutagenesis shows that DNA-binding defective mutants of AIF fail to induce cell death while retaining nuclear translocation. The potential DNA-binding site identified from mutagenesis also coincides with computational docking of a DNA duplex. These observations suggest that AIF-induced nuclear apoptosis requires a direct interaction with DNA.
+The line below this paragraph, {{ABSTRACT_PUBMED_12198487}}, adds the Publication Abstract to the page
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+{{ABSTRACT_PUBMED_12198487}}
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 [[Category: Aif]]
 [[Category: Apoptosis]]
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