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| [[Image:1lv2.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1lv2| PDB=1lv2 | SCENE= }} | | {{STRUCTURE_1lv2| PDB=1lv2 | SCENE= }} |
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| '''Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids'''
| | ===Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids=== |
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| ==Overview==
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| The 2.7 A X-ray crystal structure of the HNF4gamma ligand binding domain (LBD) revealed the presence of a fatty acid within the pocket, with the AF2 helix in a conformation characteristic of a transcriptionally active nuclear receptor. GC/MS and NMR analysis of chloroform/methanol extracts from purified HNF4alpha and HNF4gamma LBDs identified mixtures of saturated and cis-monounsaturated C14-18 fatty acids. The purified HNF4 LBDs interacted with nuclear receptor coactivators, and both HNF4 subtypes show high constitutive activity in transient transfection assays, which was reduced by mutations designed to interfere with fatty acid binding. The endogenous fatty acids did not readily exchange with radiolabeled palmitic acid, and all attempts to displace them without denaturing the protein failed. Our results suggest that the HNF4s may be transcription factors that are constitutively bound to fatty acids. | | The line below this paragraph, {{ABSTRACT_PUBMED_12220494}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12220494 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12220494}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Nuclear receptor]] | | [[Category: Nuclear receptor]] |
| [[Category: Transcription factor]] | | [[Category: Transcription factor]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:19:14 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 22:23:12 2008'' |