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| [[Image:1lm1.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1lm1| PDB=1lm1 | SCENE= }} | | {{STRUCTURE_1lm1| PDB=1lm1 | SCENE= }} |
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| '''Structural studies on the synchronization of catalytic centers in glutamate synthase: native enzyme'''
| | ===Structural studies on the synchronization of catalytic centers in glutamate synthase: native enzyme=== |
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| ==Overview==
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| The complex iron-sulfur flavoprotein glutamate synthase (GltS) plays a prominent role in ammonia assimilation in bacteria, yeasts, and plants. GltS catalyzes the formation of two molecules of l-glutamate from 2-oxoglutarate and l-glutamine via intramolecular channeling of ammonia. GltS has the impressive ability of synchronizing its distinct catalytic centers to avoid wasteful consumption of l-glutamine. We have determined the crystal structure of the ferredoxin-dependent GltS in several ligation and redox states. The structures reveal the crucial elements in the synchronization between the glutaminase site and the 2-iminoglutarate reduction site. The structural data combined with the catalytic properties of GltS indicate that binding of ferredoxin and 2-oxoglutarate to the FMN-binding domain of GltS induce a conformational change in the loop connecting the two catalytic centers. The rearrangement induces a shift in the catalytic elements of the amidotransferase domain, such that it becomes activated. This machinery, over a distance of more than 30 A, controls the ability of the enzyme to bind and hydrolyze the ammonia-donating substrate l-glutamine. | | The line below this paragraph, {{ABSTRACT_PUBMED_11967268}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11967268 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11967268}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Channeling]] | | [[Category: Channeling]] |
| [[Category: Glutamate synthase]] | | [[Category: Glutamate synthase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:03:05 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 21:24:11 2008'' |