The three-dimensional structure of protein kinase C interacting protein 1 (PKCI-1) has been solved to high resolution by x-ray crystallography using single isomorphous replacement with anomalous scattering. The gene encoding human PKCI-1 was cloned from a cDNA library by using a partial sequence obtained from interactions identified in the yeast two-hybrid system between PKCI-1 and the regulatory domain of protein kinase C-beta. The PKCI-1 protein was expressed in Pichia pastoris as a dimer of two 13.7-kDa polypeptides. PKCI-1 is a member of the HIT family of proteins, shown by sequence identity to be conserved in a broad range of organisms including mycoplasma, plants, and humans. Despite the ubiquity of this protein sequence in nature, no distinct function has been shown for the protein product in vitro or in vivo. The PKCI-1 protomer has an alpha+beta meander fold containing a five-stranded antiparallel sheet and two helices. Two protomers come together to form a 10-stranded antiparallel sheet with extensive contacts between a helix and carboxy terminal amino acids of a protomer with the corresponding amino acids in the other protomer. PKCI-1 has been shown to interact specifically with zinc. The three-dimensional structure has been solved in the presence and absence of zinc and in two crystal forms. The structure of human PKCI-1 provides a model of this family of proteins which suggests a stable fold conserved throughout nature.
The line below this paragraph, {{ABSTRACT_PUBMED_8643579}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 8643579 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_8643579}}
==About this Structure==
==About this Structure==
Line 30:
Line 34:
[[Category: Signal transduction]]
[[Category: Signal transduction]]
[[Category: Zinc binding protein]]
[[Category: Zinc binding protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:00:33 2008''
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:42:17 2008''
Revision as of 10:42, 2 July 2008
This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.
1KPB is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Three-dimensional structure of human protein kinase C interacting protein 1, a member of the HIT family of proteins., Lima CD, Klein MG, Weinstein IB, Hendrickson WA, Proc Natl Acad Sci U S A. 1996 May 28;93(11):5357-62. PMID:8643579
