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| [[Image:1kf5.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1kf5| PDB=1kf5 | SCENE= }} | | {{STRUCTURE_1kf5| PDB=1kf5 | SCENE= }} |
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| '''Atomic Resolution Structure of RNase A at pH 7.1'''
| | ===Atomic Resolution Structure of RNase A at pH 7.1=== |
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| ==Overview==
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| The diffraction pattern of protein crystals extending to atomic resolution guarantees a very accurate picture of the molecular structure and enables the study of subtle phenomena related to protein functionality. Six structures of bovine pancreatic ribonuclease at the pH* values 5.2, 5.9, 6.3, 7.1, 8.0 and 8.8 and at resolution limits in the range 1.05-1.15A have been refined. An overall description of the six structures and several aspects, mainly regarding pH-triggered conformational changes, are described here. Since subtle variations were expected, a thorough validation assessment of the six refined models was first carried out. Some stereochemical parameters, such as the N[bond]C(alpha)[bond]C angle and the pyramidalization at the carbonyl C atoms, indicate that the standard target values and their weights typically used in refinement may need revision. A detailed comparison of the six structures has provided experimental evidence on the role of Lys41 in catalysis. Furthermore, insights are given into the structural effects related to the pH-dependent binding of a sulfate anion, which mimics the phosphate group of RNA, in the active site. Finally, the results support a number of thermodynamic and kinetic experimental data concerning the role of the disulfide bridge between Cys65 and Cys72 in the folding of RNase A. | | The line below this paragraph, {{ABSTRACT_PUBMED_11856829}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11856829 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11856829}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Soaking]] | | [[Category: Soaking]] |
| [[Category: Titration]] | | [[Category: Titration]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:40:09 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:14:56 2008'' |