1jc8: Difference between revisions

Revision as of 20:00, 1 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1jc8.png

Template:STRUCTURE 1jc8

Solution structure of lactam analogue (DDap) of gp41 600-612 loop of HIVSolution structure of lactam analogue (DDap) of gp41 600-612 loop of HIV

Template:ABSTRACT PUBMED 12381305

About this StructureAbout this Structure

1JC8 is a Single protein structure. Full experimental information is available from OCA.

ReferenceReference

Structural and immunological characterisation of heteroclitic peptide analogues corresponding to the 600-612 region of the HIV envelope gp41 glycoprotein., Du AP, Limal D, Semetey V, Dali H, Jolivet M, Desgranges C, Cung MT, Briand JP, Petit MC, Muller S, J Mol Biol. 2002 Oct 25;323(3):503-21. PMID:12381305

Page seeded by OCA on Tue Jul 1 20:00:33 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1jc8.gif|left|200px]]
+{{Seed}}
+[[Image:1jc8.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_1jc8|  PDB=1jc8  |  SCENE=  }}
-'''Solution structure of lactam analogue (DDap) of gp41 600-612 loop of HIV'''
+===Solution structure of lactam analogue (DDap) of gp41 600-612 loop of HIV===
-==Overview==
+<!--
-The conformational and immunological properties of different analogues corresponding to the 600-612 disulfide loop of the human immunodeficiency virus (HIV) gp41 glycoprotein envelope were studied. Fourteen analogues were designed and synthesised; namely, a series of seven analogues in which the disulfide bond was replaced by a lactam bridge and a series of seven analogues in which one residue of each analogue at a time, was replaced by its corresponding homologised alpha-amino acid (beta(3)-amino acid). In the case of the lactam analogues, the influence of the two possible CO-NH and NH-CO orientations of the lactam bridge as well as the size of the lactam ring was explored. The analogues were tested in ELISA with monoclonal antibodies raised against the 600-612 cyclic parent peptide as well as with sera from HIV-1 infected patients. A structural analysis of the parent and analogue peptides was carried out in dimethyl sulfoxide (DMSO-d(6)) using two-dimensional NMR techniques and molecular dynamics simulations. Comparison of the own conformation of the cyclic analogues with their either strong or weak reactivity with the antibodies reveals structural features that may be correlated with the antibody reactivity. Thus, a close structural similarity, particularly a characteristic orientation of the side-chains of residues Lys606, Leu607 and Ile608 in the loop, was found in certain beta(3)-analogues that were better recognised than the parent peptide by anti-peptide mouse monoclonal antibodies and patients' antibodies.
+The line below this paragraph, {{ABSTRACT_PUBMED_12381305}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 12381305 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_12381305}}
 ==About this Structure==
-JC8 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JC8 OCA].
+JC8 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JC8 OCA].
 ==Reference==
@@ Line 35: / Line 39: @@
 [[Category: Lactam bond]]
 [[Category: Peptidomimetics.]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 21:02:38 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul  1 20:00:33 2008''