|
|
| Line 1: |
Line 1: |
| [[Image:1hji.gif|left|200px]] | | {{Seed}} |
| | [[Image:1hji.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1hji| PDB=1hji | SCENE= }} | | {{STRUCTURE_1hji| PDB=1hji | SCENE= }} |
|
| |
|
| '''BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA COMPLEX'''
| | ===BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA COMPLEX=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Nun protein from coliphage HK022 binds to phage boxB RNA and functions, in contrast to phage lambda N protein, as a transcriptional terminator. The basic Nun-(10-44) peptide contains the boxB RNA binding arginine rich motif, ARM. The peptide binds boxB RNA and competes with the phage lambda ARM peptide N-(1-36) as indicated by nuclear magnetic resonance (NMR) spectroscopy titrations. In two-dimensional nuclear Overhauser enhancement spectroscopy experiments boxB RNA in complex with Nun-(20-44) exhibits the same pattern of resonances as it does in complex with N peptides containing the ARM, and we could show that Nun-(20-44) forms a bent alpha-helix upon binding to the boxB RNA. The structure of the boxB RNA-bound Nun-(20-44) was determined on the basis of 191 intra- and 30 intermolecular distance restraints. Ser-24 is anchored to the lower RNA stem, and stacking of Tyr-39 and A7 is clearly experimentally indicated. Arg-28 shows numerous contacts to the RNA stem. Leu-22, Ile-30, Trp-33, Ile-37, and Leu-41 form a hydrophobic surface, which could be a recognition site for additional host factors such as NusG. Such a hydrophobic surface area is not present in N-(1-36) bound to boxB RNA.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11356847}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11356847 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_11356847}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| 1HJI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk022 Enterobacteria phage hk022]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJI OCA]. | | 1HJI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk022 Enterobacteria phage hk022]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJI OCA]. |
|
| |
|
| ==Reference== | | ==Reference== |
| Line 32: |
Line 36: |
| [[Category: Protein/rna]] | | [[Category: Protein/rna]] |
| [[Category: Termination]] | | [[Category: Termination]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:54:38 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:16:18 2008'' |