1hj8: Difference between revisions

Revision as of 08:15, 1 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1hj8.png

Template:STRUCTURE 1hj8

1.00 AA TRYPSIN FROM ATLANTIC SALMON1.00 AA TRYPSIN FROM ATLANTIC SALMON

Template:ABSTRACT PUBMED 11264577

About this StructureAbout this Structure

1HJ8 is a Single protein structure of sequence from Salmo salar. Full crystallographic information is available from OCA.

ReferenceReference

Atomic resolution structures of trypsin provide insight into structural radiation damage., Leiros HK, McSweeney SM, Smalas AO, Acta Crystallogr D Biol Crystallogr. 2001 Apr;57(Pt 4):488-97. PMID:11264577

Page seeded by OCA on Tue Jul 1 08:15:31 2008

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OCA

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-'''1.00 AA TRYPSIN FROM ATLANTIC SALMON'''
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-==Overview==
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-Radiation damage is an inherent problem in protein X-ray crystallography and the process has recently been shown to be highly specific, exhibiting features such as cleavage of disulfide bonds, decarboxylation of acidic residues, increase in atomic B factors and increase in unit-cell volume. Reported here are two trypsin structures at atomic resolution (1.00 and 0.95 A), the data for which were collected at a third-generation synchrotron (ESRF) at two different beamlines. Both trypsin structures exhibit broken disulfide bonds; in particular, the bond from Cys191 to Cys220 is very sensitive to synchrotron radiation. The data set collected at the most intense beamline (ID14-EH4) shows increased structural radiation damage in terms of lower occupancies for cysteine residues, more breakage in the six disulfide bonds and more alternate conformations. It appears that high intensity and not only the total X-ray dose is most harmful to protein crystals.
+The line below this paragraph, {{ABSTRACT_PUBMED_11264577}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 11264577 is the PubMed ID number.
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+{{ABSTRACT_PUBMED_11264577}}
 ==About this Structure==
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 [[Category: Salmon]]
 [[Category: Trypsin]]
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