1fzr: Difference between revisions

Revision as of 04:11, 1 July 2008

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File:1fzr.png

Template:STRUCTURE 1fzr

CRYSTAL STRUCTURE OF BACTERIOPHAGE T7 ENDONUCLEASE ICRYSTAL STRUCTURE OF BACTERIOPHAGE T7 ENDONUCLEASE I

Template:ABSTRACT PUBMED 11135673

About this StructureAbout this Structure

1FZR is a Single protein structure of sequence from Enterobacteria phage t7. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the Holliday junction resolving enzyme T7 endonuclease I., Hadden JM, Convery MA, Declais AC, Lilley DM, Phillips SE, Nat Struct Biol. 2001 Jan;8(1):62-7. PMID:11135673

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-'''CRYSTAL STRUCTURE OF BACTERIOPHAGE T7 ENDONUCLEASE I'''
+===CRYSTAL STRUCTURE OF BACTERIOPHAGE T7 ENDONUCLEASE I===
-==Overview==
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-We have solved the crystal structure of the Holliday junction resolving enzyme T7 endonuclease I at 2.1 A resolution using the multiwavelength anomalous dispersion (MAD) technique. Endonuclease I exhibits strong structural specificity for four-way DNA junctions. The structure shows that it forms a symmetric homodimer arranged in two well-separated domains. Each domain, however, is composed of elements from both subunits, and amino acid side chains from both protomers contribute to the active site. While no significant structural similarity could be detected with any other junction resolving enzyme, the active site is similar to that found in several restriction endonucleases. T7 endonuclease I therefore represents the first crystal structure of a junction resolving enzyme that is a member of the nuclease superfamily of enzymes.
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+{{ABSTRACT_PUBMED_11135673}}
 ==About this Structure==
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 [[Category: Holliday junction resolvase]]
 [[Category: Homodimer]]
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