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| {{STRUCTURE_1ft6| PDB=1ft6 | SCENE= }} | | {{STRUCTURE_1ft6| PDB=1ft6 | SCENE= }} |
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| '''REDUCED STATE OF CYTOCHROME C554 FROM NITROSOMONAS EUROPAEA'''
| | ===REDUCED STATE OF CYTOCHROME C554 FROM NITROSOMONAS EUROPAEA=== |
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| ==Overview==
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| Cytochrome c554 (cyt c554) is a tetra-heme cytochrome involved in the oxidation of NH3 by Nitrosomonas europaea. The X-ray crystal structures of both the oxidized and dithionite-reduced states of cyt c554 in a new, rhombohedral crystal form have been solved by molecular replacement, at 1.6 A and 1.8 A resolution, respectively. Upon reduction, the conformation of the polypeptide chain changes between residues 175 and 179, which are adjacent to hemes III and IV. Cyt c554 displays conserved heme-packing motifs that are present in other heme-containing proteins. Comparisons to hydroxylamine oxidoreductase, the electron donor to cyt c554, and cytochrome c nitrite reductase, an enzyme involved in nitrite ammonification, reveal substantial structural similarity in the polypeptide chain surrounding the heme core environment. The structural determinants of these heme-packing motifs extend to the buried water molecules that hydrogen bond to the histidine ligands to the heme iron. In the original structure determination of a tetragonal crystal form, a cis peptide bond between His129 and Phe130 was identified that appeared to be stabilized by crystal contacts. In the rhombohedral crystal form used in the present high-resolution structure determination, this peptide bond adopts the trans conformation, but with disallowed angles of phi and psi.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11372197}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11372197 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11372197}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Rees, D C.]] | | [[Category: Rees, D C.]] |
| [[Category: Heme-stacking]] | | [[Category: Heme-stacking]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:44:20 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:54:42 2008'' |