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| {{STRUCTURE_1fgs| PDB=1fgs | SCENE= }} | | {{STRUCTURE_1fgs| PDB=1fgs | SCENE= }} |
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| '''FOLYLPOLYGLUTAMATE SYNTHETASE FROM LACTOBACILLUS CASEI'''
| | ===FOLYLPOLYGLUTAMATE SYNTHETASE FROM LACTOBACILLUS CASEI=== |
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| ==Overview==
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| Folylpolyglutamate synthetase, which is responsible for the addition of a polyglutamate tail to folate and folate derivatives, is an ATP-dependent enzyme isolated from eukaryotic and bacterial sources, where it plays a key role in the retention of the intracellular folate pool. Here, we report the 2.4-A resolution crystal structure of the MgATP complex of the enzyme from Lactobacillus casei. The structural analysis reveals that folylpolyglutamate synthetase is a modular protein consisting of two domains, one with a typical mononucleotide-binding fold and the other strikingly similar to the folate-binding enzyme dihydrofolate reductase. We have located the active site of the enzyme in a large interdomain cleft adjacent to an ATP-binding P-loop motif. Opposite this site, in the C domain, a cavity likely to be the folate binding site has been identified, and inspection of this cavity and the surrounding protein structure suggests that the glutamate tail of the substrate may project into the active site. A further feature of the structure is a well defined Omega loop, which contributes both to the active site and to interdomain interactions. The determination of the structure of this enzyme represents the first step toward the elucidation of the molecular mechanism of polyglutamylation of folates and antifolates.
| | The line below this paragraph, {{ABSTRACT_PUBMED_9618466}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 9618466 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_9618466}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Ligase]] | | [[Category: Ligase]] |
| [[Category: Synthetase]] | | [[Category: Synthetase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:18:31 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:13:36 2008'' |