1f1w: Difference between revisions

Revision as of 02:21, 1 July 2008

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File:1f1w.png

Template:STRUCTURE 1f1w

SRC SH2 THREF1TRP MUTANT COMPLEXED WITH THE PHOSPHOPEPTIDE S(PTR)VNVQNSRC SH2 THREF1TRP MUTANT COMPLEXED WITH THE PHOSPHOPEPTIDE S(PTR)VNVQN

Template:ABSTRACT PUBMED 10911998

About this StructureAbout this Structure

1F1W is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for specificity switching of the Src SH2 domain., Kimber MS, Nachman J, Cunningham AM, Gish GD, Pawson T, Pai EF, Mol Cell. 2000 Jun;5(6):1043-9. PMID:10911998

Page seeded by OCA on Tue Jul 1 02:21:09 2008

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-[[Image:1f1w.gif|left|200px]]
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 {{STRUCTURE_1f1w|  PDB=1f1w  |  SCENE=  }}
-'''SRC SH2 THREF1TRP MUTANT COMPLEXED WITH THE PHOSPHOPEPTIDE S(PTR)VNVQN'''
+===SRC SH2 THREF1TRP MUTANT COMPLEXED WITH THE PHOSPHOPEPTIDE S(PTR)VNVQN===
-==Overview==
+<!--
-The Src SH2 domain binds pYEEI-containing phosphopeptides in an extended conformation with a hydrophobic pocket, which includes ThrEF1, binding Ile(pY +3). Mutating ThrEF1 to tryptophan switches specificity to an Asn(pY +2) requirement, yielding a biological mimic of the Grb2 SH2 domain. Here we show that the Src ThrEF1Trp SH2 domain mutant binds pYVNV phosphopeptides in a beta turn conformation, which, despite differing conformations of the interacting tryptophan, closely resembles the native Grb2/pYVNV cognate peptide binding mode. The ThrEF1Trp substitution therefore switches specificity by physically occluding the pTyr +3 binding pocket and by providing additional interaction surface area for Asn(pY +2). This demonstrates structurally how novel SH2 domain specificities may rapidly evolve through single amino acid substitutions and suggests how new signaling pathways may develop.
+The line below this paragraph, {{ABSTRACT_PUBMED_10911998}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 10911998 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_10911998}}
 ==About this Structure==
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 [[Category: Specificity switch]]
 [[Category: Src]]
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