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| [[Image:1el0.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1el0| PDB=1el0 | SCENE= }} | | {{STRUCTURE_1el0| PDB=1el0 | SCENE= }} |
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| '''SOLUTION STRUCTURE OF THE HUMAN CC CHEMOKINE, I-309'''
| | ===SOLUTION STRUCTURE OF THE HUMAN CC CHEMOKINE, I-309=== |
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| ==Overview==
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| I-309 is a member of the CC subclass of chemokines and is one of only three human chemokines known to contain an additional, third disulfide bond. The three-dimensional solution structure of I-309 was determined by (1)H nuclear magnetic resonance spectroscopy and dynamic simulated annealing. The structure of I-309, which remains monomeric at high concentrations, was determined on the basis of 978 experimental restraints. The N-terminal region of I-309 was disordered, as has been previously observed for the CC chemokine eotaxin but not others such as MCP-1 and RANTES. This was followed in I-309 by a well-ordered region between residues 13 and 69 that consisted of a 3(10)-helix, a triple-stranded antiparallel beta-sheet, and finally a C-terminal alpha-helix. Root-mean-square deviations of 0.61 and 1.16 were observed for the backbone and heavy atoms, respectively. A comparison of I-309 to eotaxin and HCC-2 revealed a significant structural change in the C-terminal region of the protein. The alpha-helix normally present in chemokines was terminated early and was followed by a short section of extended strand. These changes were a direct result of the additional disulfide bond present in this protein. An examination of the I-309 structure will aid in an understanding of the specificity of this protein with its receptor, CCR8.
| | The line below this paragraph, {{ABSTRACT_PUBMED_10821677}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10821677 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_10821677}} |
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| ==About this Structure== | | ==About this Structure== |
| 1EL0 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EL0 OCA]. | | 1EL0 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EL0 OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Sykes, B D.]] | | [[Category: Sykes, B D.]] |
| [[Category: Chemokine fold]] | | [[Category: Chemokine fold]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:13:48 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:55:05 2008'' |