1eh8: Difference between revisions

Revision as of 00:41, 1 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1eh8.png

Template:STRUCTURE 1eh8

BENZYLATED HUMAN O6-ALKYLGUANINE-DNA ALKYLTRANSFERASEBENZYLATED HUMAN O6-ALKYLGUANINE-DNA ALKYLTRANSFERASE

Template:ABSTRACT PUBMED 10747039

About this StructureAbout this Structure

1EH8 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Active and alkylated human AGT structures: a novel zinc site, inhibitor and extrahelical base binding., Daniels DS, Mol CD, Arvai AS, Kanugula S, Pegg AE, Tainer JA, EMBO J. 2000 Apr 3;19(7):1719-30. PMID:10747039

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-[[Image:1eh8.gif|left|200px]]
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 {{STRUCTURE_1eh8|  PDB=1eh8  |  SCENE=  }}
-'''BENZYLATED HUMAN O6-ALKYLGUANINE-DNA ALKYLTRANSFERASE'''
+===BENZYLATED HUMAN O6-ALKYLGUANINE-DNA ALKYLTRANSFERASE===
-==Overview==
+<!--
-Human O(6)-alkylguanine-DNA alkyltransferase (AGT), which directly reverses endogenous alkylation at the O(6)-position of guanine, confers resistance to alkylation chemotherapies and is therefore an active anticancer drug target. Crystal structures of active human AGT and its biologically and therapeutically relevant methylated and benzylated product complexes reveal an unexpected zinc-stabilized helical bridge joining a two-domain alpha/beta structure. An asparagine hinge couples the active site motif to a helix-turn-helix (HTH) motif implicated in DNA binding. The reactive cysteine environment, its position within a groove adjacent to the alkyl-binding cavity and mutational analyses characterize DNA-damage recognition and inhibitor specificity, support a structure-based dealkylation mechanism and suggest a molecular basis for destabilization of the alkylated protein. These results support damaged nucleotide flipping facilitated by an arginine finger within the HTH motif to stabilize the extrahelical O(6)-alkylguanine without the protein conformational change originally proposed from the empty Ada structure. Cysteine alkylation sterically shifts the HTH recognition helix to evidently mechanistically couple release of repaired DNA to an opening of the protein fold to promote the biological turnover of the alkylated protein.
+The line below this paragraph, {{ABSTRACT_PUBMED_10747039}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 10747039 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_10747039}}
 ==About this Structure==
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 [[Category: Dna repair]]
 [[Category: Methyltransferase]]
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