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| [[Image:1e1u.jpg|left|200px]] | | {{Seed}} |
| | [[Image:1e1u.png|left|200px]] |
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| {{STRUCTURE_1e1u| PDB=1e1u | SCENE= }} | | {{STRUCTURE_1e1u| PDB=1e1u | SCENE= }} |
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| '''HUMAN PRION PROTEIN VARIANT R220K'''
| | ===HUMAN PRION PROTEIN VARIANT R220K=== |
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| ==Overview==
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| The NMR structures of three single-amino acid variants of the C-terminal domain of the human prion protein, hPrP(121-230), are presented. In hPrP(M166V) and hPrP(R220K) the substitution is with the corresponding residue in murine PrP, and in hPrP(S170N) it is with the corresponding Syrian hamster residue. All three substitutions are in the surface region of the structure of the cellular form of PrP (PrP(C)) that is formed by the C-terminal part of helix 3, with residues 218-230, and a loop of residues 166-172. This molecular region shows high species variability and has been implicated in specific interactions with a so far not further characterized "protein X," and it is related to the species barrier for transmission of prion diseases. As expected, the three variant hPrP(121-230) structures have the same global architecture as the previously determined wild-type bovine, human, murine, and Syrian hamster prion proteins, but with the present study two localized "conformational markers" could be related with single amino acid exchanges. These are the length and quality of definition of helix 3, and the NMR-observability of the residues in the loop 166-172. Poor definition of the C-terminal part of helix 3 is characteristic for murine PrP and has now been observed also for hPrP(R220K), and NMR observation of the complete loop 166-172 has so far been unique for Syrian hamster PrP and is now also documented for hPrP(S170N). | | The line below this paragraph, {{ABSTRACT_PUBMED_10900000}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10900000 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_10900000}} |
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| ==About this Structure== | | ==About this Structure== |
| 1E1U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E1U OCA]. | | 1E1U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E1U OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Zahn, R.]] | | [[Category: Zahn, R.]] |
| [[Category: Prion protein]] | | [[Category: Prion protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:33:17 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:00:13 2008'' |