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| [[Image:1duq.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1duq| PDB=1duq | SCENE= }} | | {{STRUCTURE_1duq| PDB=1duq | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF THE REV BINDING ELEMENT OF HIV-1'''
| | ===CRYSTAL STRUCTURE OF THE REV BINDING ELEMENT OF HIV-1=== |
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| ==Overview==
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| The crystal and molecular structure of an RNA duplex corresponding to the high affinity Rev protein binding element (RBE) has been determined at 2.1-A resolution. Four unique duplexes are present in the crystal, comprising two structural variants. In each duplex, the RNA double helix consists of an annealed 12-mer and 14-mer that form an asymmetric internal loop consisting of G-G and G-A noncanonical base pairs and a flipped-out uridine. The 12-mer strand has an A-form conformation, whereas the 14-mer strand is distorted to accommodate the bulges and noncanonical base pairing. In contrast to the NMR model of the unbound RBE, an asymmetric G-G pair with N2-N7 and N1-O6 hydrogen bonding, is formed in each helix. The G-A base pairing agrees with the NMR structure in one structural variant, but forms a novel water-mediated pair in the other. A backbone flip and reorientation of the G-G base pair is required to assume the RBE conformation present in the NMR model of the complex between the RBE and the Rev peptide. | | The line below this paragraph, {{ABSTRACT_PUBMED_10792052}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10792052 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_10792052}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Rev binding domain]] | | [[Category: Rev binding domain]] |
| [[Category: Rre]] | | [[Category: Rre]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:18:06 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:38:49 2008'' |