1d3n: Difference between revisions

Revision as of 22:19, 30 June 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1d3n.png

Template:STRUCTURE 1d3n

METHIONINE CORE MUTATIONMETHIONINE CORE MUTATION

Template:ABSTRACT PUBMED 10666571

About this StructureAbout this Structure

1D3N is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

ReferenceReference

Use of differentially substituted selenomethionine proteins in X-ray structure determination., Gassner NC, Matthews BW, Acta Crystallogr D Biol Crystallogr. 1999 Dec;55(Pt 12):1967-70. PMID:10666571

Page seeded by OCA on Mon Jun 30 22:19:22 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-'''METHIONINE CORE MUTATION'''
+===METHIONINE CORE MUTATION===
-==Overview==
+<!--
-Using heavily methionine-substituted T4 lysozyme as an example, it is shown how the addition or deletion of a small number of methionines can simplify the location of selenium sites for use in MAD phasing. By comparing the X-ray data for a large number of singly substituted lysozymes, it is shown that the optimal amino acid to be substituted by methionine is leucine, followed, in order of preference, by phenylalanine, isoleucine and valine. The identification of leucine as the first choice agrees with the ranking suggested by the Dayhoff mutation probability, i.e. by the frequency of amino-acid substitutions in the sequences of related proteins. The ranking of the second and subsequent choices, however, differ significantly.
+The line below this paragraph, {{ABSTRACT_PUBMED_10666571}}, adds the Publication Abstract to the page
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+{{ABSTRACT_PUBMED_10666571}}
 ==About this Structure==
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 [[Category: Selenomethionine]]
 [[Category: T4 lysozyme]]
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