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| {{STRUCTURE_1cm0| PDB=1cm0 | SCENE= }} | | {{STRUCTURE_1cm0| PDB=1cm0 | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF THE PCAF/COENZYME-A COMPLEX'''
| | ===CRYSTAL STRUCTURE OF THE PCAF/COENZYME-A COMPLEX=== |
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| ==Overview==
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| The human p300/CBP-associating factor, PCAF, mediates transcriptional activation through its ability to acetylate nucleosomal histone substrates as well as transcriptional activators such as p53. We have determined the 2.3 A crystal structure of the histone acetyltransferase (HAT) domain of PCAF bound to coenzyme A. The structure reveals a central protein core associated with coenzyme A binding and a pronounced cleft that sits over the protein core and is flanked on opposite sides by the N- and C-terminal protein segments. A correlation of the structure with the extensive mutagenesis data for PCAF and the homologous yeast GCN5 protein implicates the cleft and the N- and C-terminal protein segments as playing an important role in histone substrate binding, and a glutamate residue in the protein core as playing an essential catalytic role. A structural comparison with the coenzyme-bound forms of the related N-acetyltransferases, HAT1 (yeast histone acetyltransferase 1) and SmAAT (Serratia marcescens aminoglycoside 3-N-acetyltransferase), suggests the mode of substrate binding and catalysis by these enzymes and establishes a paradigm for understanding the structure-function relationships of other enzymes that acetylate histones and transcriptional regulators to promote activated transcription. | | The line below this paragraph, {{ABSTRACT_PUBMED_10393169}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10393169 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_10393169}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Coenzyme some]] | | [[Category: Coenzyme some]] |
| [[Category: P300/cbp associated factor]] | | [[Category: P300/cbp associated factor]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:52:44 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:56:05 2008'' |