1c7v: Difference between revisions

Revision as of 20:20, 30 June 2008

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File:1c7v.png

Template:STRUCTURE 1c7v

NMR SOLUTION STRUCTURE OF THE CALCIUM-BOUND C-TERMINAL DOMAIN (W81-S161) OF CALCIUM VECTOR PROTEIN FROM AMPHIOXUSNMR SOLUTION STRUCTURE OF THE CALCIUM-BOUND C-TERMINAL DOMAIN (W81-S161) OF CALCIUM VECTOR PROTEIN FROM AMPHIOXUS

Template:ABSTRACT PUBMED 10891072

About this StructureAbout this Structure

1C7V is a Single protein structure of sequence from Branchiostoma lanceolatum. Full experimental information is available from OCA.

ReferenceReference

Sequential calcium binding to the regulatory domain of calcium vector protein reveals functional asymmetry and a novel mode of structural rearrangement., Theret I, Baladi S, Cox JA, Sakamoto H, Craescu CT, Biochemistry. 2000 Jul 11;39(27):7920-6. PMID:10891072

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OCA

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-[[Image:1c7v.gif|left|200px]]
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-'''NMR SOLUTION STRUCTURE OF THE CALCIUM-BOUND C-TERMINAL DOMAIN (W81-S161) OF CALCIUM VECTOR PROTEIN FROM AMPHIOXUS'''
+===NMR SOLUTION STRUCTURE OF THE CALCIUM-BOUND C-TERMINAL DOMAIN (W81-S161) OF CALCIUM VECTOR PROTEIN FROM AMPHIOXUS===
-==Overview==
+<!--
-Calcium vector protein (CaVP) from amphioxus is a two-domain, calcium-binding protein (18.3 kDa) of the calmodulin superfamily. Only two of the four EF-hand motifs (sites III and IV) have a significant binding affinity for calcium ions. We determined the solution structure of the domain containing these active sites (C-CaVP: W81-S161), in the Ca(2+)-saturated state, using NMR spectroscopy and restrained molecular dynamics. The tertiary structure is similar to other Ca(2+)-binding domains containing a pair of EF-hand motifs. The apo state has spectroscopic and thermodynamic characteristics of a molten globule, with conserved secondary structure but highly fluctuating tertiary organization. Titration of C-CaVP with Ca(2+) revealed a stepwise ion binding, with a stable equilibrium intermediate in which only site III binds a calcium ion. Despite a highly fluctuating structure of the free site IV, the calcium-bound site III has a persistent structure, with similar secondary elements but different interhelix angle and hydrophobic packing relative to the fully calcium-saturated state.
+The line below this paragraph, {{ABSTRACT_PUBMED_10891072}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 10891072 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_10891072}}
 ==About this Structure==
-C7V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Branchiostoma_lanceolatum Branchiostoma lanceolatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C7V OCA].
+C7V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Branchiostoma_lanceolatum Branchiostoma lanceolatum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C7V OCA].
 ==Reference==
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 [[Category: Ef-hand family]]
 [[Category: Nmr]]
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