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| {{STRUCTURE_1bi2| PDB=1bi2 | SCENE= }} | | {{STRUCTURE_1bi2| PDB=1bi2 | SCENE= }} |
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| '''STRUCTURE OF APO-AND HOLO-DIPHTHERIA TOXIN REPRESSOR'''
| | ===STRUCTURE OF APO-AND HOLO-DIPHTHERIA TOXIN REPRESSOR=== |
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| ==Overview==
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| The diphtheria toxin repressor (DtxR) from Corynebacterium diphtheriae is a divalent metal-activated repressor of chromosomal genes that encode proteins responsible for siderophore-mediated iron uptake and also of the gene of certain corynebacteriophages that encodes diphtheria toxin. DtxR consists of two 25.3-kDa three-domain subunits and is a member of a family of related repressor proteins in several Gram-positive bacterial species, some of which are important human pathogens. In this paper, we report on the first high resolution crystal structures of apo-DtxR in two related space groups. In addition, crystal structures of Zn-DtxR were determined in the same two space groups. The resolutions of the structures range from 2.2 to 2.4 A. The four refined models of the apo- and the holo-repressor exhibit quite similar metal binding centers, which do, however, show higher thermal motion in the apo-structures. All four structures reported differ from each other in one important aspect. The N-terminal DNA-binding domain and the last 20 residues of the dimerization domain of each subunit move significantly with respect to the core of the DtxR dimer, which consists of residues 74-120 from both subunits. These results provide the first indication of a conformational change that may occur upon binding of the holo-repressor to DNA. | | The line below this paragraph, {{ABSTRACT_PUBMED_9712865}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 9712865 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_9712865}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Repressor]] | | [[Category: Repressor]] |
| [[Category: Transcription regulation]] | | [[Category: Transcription regulation]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:32:24 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:11:52 2008'' |