1b50: Difference between revisions

Revision as of 18:16, 30 June 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1b50.png

Template:STRUCTURE 1b50

NMR STRUCTURE OF HUMAN MIP-1A D26A, 10 STRUCTURESNMR STRUCTURE OF HUMAN MIP-1A D26A, 10 STRUCTURES

Template:ABSTRACT PUBMED 10347159

About this StructureAbout this Structure

1B50 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

ReferenceReference

Identification of amino acid residues critical for aggregation of human CC chemokines macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, and RANTES. Characterization of active disaggregated chemokine variants., Czaplewski LG, McKeating J, Craven CJ, Higgins LD, Appay V, Brown A, Dudgeon T, Howard LA, Meyers T, Owen J, Palan SR, Tan P, Wilson G, Woods NR, Heyworth CM, Lord BI, Brotherton D, Christison R, Craig S, Cribbes S, Edwards RM, Evans SJ, Gilbert R, Morgan P, Randle E, Schofield N, Varley PG, Fisher J, Waltho JP, Hunter MG, J Biol Chem. 1999 Jun 4;274(23):16077-84. PMID:10347159

Page seeded by OCA on Mon Jun 30 18:16:46 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1b50.gif|left|200px]]
+{{Seed}}
+[[Image:1b50.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_1b50|  PDB=1b50  |  SCENE=  }}
-'''NMR STRUCTURE OF HUMAN MIP-1A D26A, 10 STRUCTURES'''
+===NMR STRUCTURE OF HUMAN MIP-1A D26A, 10 STRUCTURES===
-==Overview==
+<!--
-Human CC chemokines macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, and RANTES (regulated on activation normal T cell expressed) self-associate to form high-molecular mass aggregates. To explore the biological significance of chemokine aggregation, nonaggregating variants were sought. The phenotypes of 105 hMIP-1alpha variants generated by systematic mutagenesis and expression in yeast were determined. hMIP-1alpha residues Asp26 and Glu66 were critical to the self-association process. Substitution at either residue resulted in the formation of essentially homogenous tetramers at 0.5 mg/ml. Substitution of identical or analogous residues in homologous positions in both hMIP-1beta and RANTES demonstrated that they were also critical to aggregation. Our analysis suggests that a single charged residue at either position 26 or 66 is insufficient to support extensive aggregation and that two charged residues must be present. Solution of the three-dimensional NMR structure of hMIP-1alpha has enabled comparison of these residues in hMIP-1beta and RANTES. Aggregated and disaggregated forms of hMIP-1alpha, hMIP-1beta, and RANTES generally have equivalent G-protein-coupled receptor-mediated biological potencies. We have therefore generated novel reagents to evaluate the role of hMIP-1alpha, hMIP-1beta, and RANTES aggregation in vitro and in vivo. The disaggregated chemokines retained their human immunodeficiency virus (HIV) inhibitory activities. Surprisingly, high concentrations of RANTES, but not disaggregated RANTES variants, enhanced infection of cells by both M- and T-tropic HIV isolates/strains. This observation has important implications for potential therapeutic uses of chemokines implying that disaggregated forms may be necessary for safe clinical investigation.
+The line below this paragraph, {{ABSTRACT_PUBMED_10347159}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 10347159 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_10347159}}
 ==About this Structure==
-B50 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B50 OCA].
+B50 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B50 OCA].
 ==Reference==
@@ Line 30: / Line 34: @@
 [[Category: Chemotaxis]]
 [[Category: Cytokine]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:04:52 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:16:46 2008''