1a7d: Difference between revisions

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{{Seed}}
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{{STRUCTURE_1a7d|  PDB=1a7d  |  SCENE=  }}  
{{STRUCTURE_1a7d|  PDB=1a7d  |  SCENE=  }}  


'''CHLOROMET MYOHEMERYTHRIN FROM THEMISTE ZOSTERICOLA'''
===CHLOROMET MYOHEMERYTHRIN FROM THEMISTE ZOSTERICOLA===




==Overview==
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Myohemerythrin (Mhr) is a nonheme iron oxygen carrier found in the retractor muscles of marine "peanut" worms. The X-ray crystal structures of two recombinant Themiste zostericola Mhrs are reported to a resolution of 1.8 A. Surprisingly, the met wild-type structure (R = 17.8%) was found to contain chloride bound to Fe2, while coordinated hydroxide was found in the met L103N structure (R = 18.3%). An internal water molecule was also found distal to the Fe-O-Fe center of the mutant protein, forming hydrogen bonds with the coordinated hydroxide and the OD1 atom of Asn-103. This finding is consistent with the kinetic and spectroscopic results reported for the L103N mutant Mhr [Raner, G. M., Martins, L. J., &amp; Ellis, W. R., Jr. (1997) Biochemistry 36, 7037-7043]. Possible roles for the side chain of residue 103 (Leu in wild-type Mhr) in gating ligand binding are also discussed.
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{{ABSTRACT_PUBMED_9188702}}


==About this Structure==
==About this Structure==
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[[Category: Nonheme iron oxygen carrier]]
[[Category: Nonheme iron oxygen carrier]]
[[Category: Oxygen transport]]
[[Category: Oxygen transport]]
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