2ncd: Difference between revisions

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-[[Image:2ncd.gif|left|200px]]
+[[Image:2ncd.png|left|200px]]
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-==Overview==
+{{ABSTRACT_9796817}}
-Motor proteins of the kinesin superfamily transport intracellular cargo along microtubules. Although different kinesin proteins share 30-50% amino-acid identity in their motor catalytic cores, some move to the plus end of microtubules whereas others travel in the opposite direction. Crystal structures of the catalytic cores of conventional kinesin (a plus-end-directed motor involved in organelle transport) and ncd (a minus-end-directed motor involved in chromosome segregation) are nearly identical; therefore, the structural basis for their opposite directions of movement is unknown. Here we show that the ncd 'neck' made up of 13 class-specific residues next to the superfamily-conserved catalytic core, is essential for minus-end-directed motility, as mutagenesis of these neck residues reverses the direction of ncd motion. By solving the 2.5 A structure of a functional ncd dimer, we show that the ncd neck (a coiled-coil) differs from the corresponding region in the kinesin neck (an interrupted beta-strand), although both necks interact with similar elements in the catalytic cores. The distinct neck architectures also confer different symmetries to the ncd and kinesin dimers and position these motors with appropriate directional bias on the microtubule.
 ==About this Structure==
 NCD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. The following page contains interesting information on the relation of 2NCD with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb64_1.html Kinesin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NCD OCA].
-==Reference==
-Direction determination in the minus-end-directed kinesin motor ncd., Sablin EP, Case RB, Dai SC, Hart CL, Ruby A, Vale RD, Fletterick RJ, Nature. 1998 Oct 22;395(6704):813-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9796817 9796817]
 [[Category: Drosophila melanogaster]]
 [[Category: Kinesin]]
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 [[Category: Microtubule-based motor]]
 [[Category: Ncd]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 09:35:32 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 18:20:27 2008''